LOCUS       sequence-129330-        1189 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_895_Nhe2_EcR-to-gl
FEATURES             Location/Qualifiers
     source          1..1189
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..52
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(474..498)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      689..698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(761..779)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 cagaacattt ctctatcgat aggtaccaca agtttgtaca aaaaagcagg cttcgttttc
       61 aatttcaaga atatgtcaac actctgtaat catttcaaga ataaaattaa aacaccaaat
      121 tgaatttgtg gaaggcctag gccactggtt agaacaattg aatatggaga cggaaaaatg
      181 catgaggcat catcctaaat tgactaccaa ttttatataa tgagatgggc gacactcatt
      241 ccagcagata gcgaatacca acggatgcaa ttgctcaagt cgggacaatt actgaatccg
      301 acatttcaag aataagagat ttatcagcat ctcgaaaatt acactcgatg tacacaccaa
      361 ttgctcaatt gggagtcttt agtcattccg cactcgacag ataagctttc gctactgctg
      421 ctaataaaat ttcgccaata atgcaaatca gatcttatcg caggccaact gggacccagc
      481 tttcttgtac aaagtggtag atctgcgatc gagctcgccc ggggatcgag cgcagcggta
      541 taaaagggcg cggggtggct gagagcatca gttgtgaatg aatgttcgag ccgagcagac
      601 gtgccgctgc cttcgttaat atcctttgaa taagccaact ttgaatcaca agacgcatac
      661 caaacggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa acataaagaa
      721 aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc
      781 tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt
      841 ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg
      901 atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt
      961 tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta
     1021 taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc
     1081 caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat
     1141 tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtac
//
