LOCUS       sequence-129331-        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_895_Nhe2_EcR-to-gl
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    239..263
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(685..709)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    723..814
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(755..774)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    828..876
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc atggtggctt taccaacagt
       61 accggaatgc cgtttggtat gcgtcttgtg attcaaagtt ggcttattca aaggatatta
      121 acgaaggcag cggcacgtct gctcggctcg aacattcatt cacaactgat gctctcagcc
      181 accccgcgcc cttttatacc gctgcgctcg atccccgggc gagctcgatc gcagatctac
      241 cactttgtac aagaaagctg ggtcccagtt ggcctgcgat aagatctgat ttgcattatt
      301 ggcgaaattt tattagcagc agtagcgaaa gcttatctgt cgagtgcgga atgactaaag
      361 actcccaatt gagcaattgg tgtgtacatc gagtgtaatt ttcgagatgc tgataaatct
      421 cttattcttg aaatgtcgga ttcagtaatt gtcccgactt gagcaattgc atccgttggt
      481 attcgctatc tgctggaatg agtgtcgccc atctcattat ataaaattgg tagtcaattt
      541 aggatgatgc ctcatgcatt tttccgtctc catattcaat tgttctaacc agtggcctag
      601 gccttccaca aattcaattt ggtgttttaa ttttattctt gaaatgatta cagagtgttg
      661 acatattctt gaaattgaaa acgaagcctg cttttttgta caaacttgtg gtacctatcg
      721 atagagaaat gttctggcac ctgcacttgc actggggaca gcctattttg ctagtttgtt
      781 ttgtttcgtt ttgttttgat ggagagcgta tgttagtact atcgattcac acaaaaaacc
      841 aacacacaga tgtaatgaaa ataaagatat tttattgcgg ccgctccaag tacctcccgt
      901 accttaatat tacttactta tcatggtagc ttgggctggc gtaatagcga agaggcccgc
      961 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcaaat tgtaagcgtt
     1021 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     1081 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttga
//
