LOCUS       sequence-129336-         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-DHFRHIS
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             494..516
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(553..572)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(554..572)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggcacgacgc tcgaggattt cgaaacaata gctatgcgag ttctgcgcat cagcatcgtg
       61 gaattcgctg aataccgatt cccagtcatc cggctcgtaa tccggaaaat gggtgtcgcc
      121 ttccacttct gcatcgatat gcgtcagata cagtttttgc gcttttggca agaactgttc
      181 ataaacgcgt ccgccgccaa tcaccatgat ttctggtacg tcaccacacg ccgcgatggc
      241 ttcgtcgacc gatttaaccc aggtaacccg atcatcggtc ccgggttgac tgctgaggat
      301 aatatttttg cggccgggca aaggcctacc gattgattcc caggtatggc gccccataat
      361 cacgggttta tttaaggtgt tgcgtttaaa ccaggcgaga tcggcaggca ggttccatgg
      421 catggcgttt tccatgccga taacgcgatc taccgctagc gccgcaatca gactgatgga
      481 tcccatatgt atatctcctt cttaaagtta aacaaaatta tttccggagc cggagggaaa
      541 ccgttgtggt ctccctatag tgagtcgtat taatttcgcg ggatcgatgc atggtgccaa
      601 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgat
//
