LOCUS       sequence-129338-        1172 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_900_Nhe2_EcR-to-gl_RB
FEATURES             Location/Qualifiers
     source          1..1172
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..52
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(474..498)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      689..698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(761..779)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 cagaacattt ctctatcgat aggtaccaca agtttgtaca aaaaagcagg cttcctgcat
       61 catttcaaga atacaggtcc attattcata aatttcaaga ataaacagag actactcgcg
      121 aatccgaaga cccaaaccga atattactat cactcattcg taaaatcaaa gttcacccca
      181 agaatcgact cgattcaagt cactaacaag gttcttacag gccttcaaaa attagactca
      241 tggattatgg gtagagccgt tgaacacttg aatgagtgtc ttaatagggt ggccacgttc
      301 gtatttcaag aatatattag gggacggtac cctggaagaa gctttcagtg caaaggacga
      361 gttaatagtt tcaatacccg gtcaataata agcaccgaca cgaaacaatt ataatgatta
      421 tacgttcttg gtctctgtta accttaatcc agataatagg tcgcaaatta aagacccagc
      481 tttcttgtac aaagtggtag atctgcgatc gagctcgccc ggggatcgag cgcagcggta
      541 taaaagggcg cggggtggct gagagcatca gttgtgaatg aatgttcgag ccgagcagac
      601 gtgccgctgc cttcgttaat atcctttgaa taagccaact ttgaatcaca agacgcatac
      661 caaacggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa acataaagaa
      721 aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc
      781 tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt
      841 ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg
      901 atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt
      961 tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta
     1021 taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc
     1081 caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat
     1141 tattatcatg gattctaaaa cggattacca gg
//
