LOCUS       sequence-129344-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-DHFRSTR
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..55)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     RBS             558..580
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(617..636)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(618..636)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agccggatca gcgcgcacgc gttgtacatt atttttcgaa ctgcgggtgg ctccaagcgc
       61 tgccggcacg acgctcgagg atttcgaaac aatagctatg cgagttctgc gcatcagcat
      121 cgtggaattc gctgaatacc gattcccagt catccggctc gtaatccgga aaatgggtgt
      181 cgccttccac ttctgcatcg atatgcgtca gatacagttt ttgcgctttt ggcaagaact
      241 gttcataaac gcgtccgccg ccaatcacca tgatttctgg tacgtcacca cacgccgcga
      301 tggcttcgtc gaccgattta acccaggtaa cccgatcatc ggtcccgggt tgactgctga
      361 ggataatatt tttgcggccg ggcaaaggcc taccgattga ttcccaggta tggcgcccca
      421 taatcacggg tttatttaag gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc
      481 atggcatggc gttttccatg ccgataacgc gatctaccgc tagcgccgca atcagactga
      541 tggatcccat atgtatatct ccttcttaaa gttaaacaaa attatttccg gagccggagg
      601 gaaaccgttg tggtctccct atagtgagtc gtattaattt cgcgggatcg atgcatggtg
      661 ccaacaccta catctgtatt aacgaagcgc tggcattgac cc
//
