LOCUS       sequence-129345-        1169 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_881_Nhe2_EcR-to-HLH4C
FEATURES             Location/Qualifiers
     source          1..1169
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    29..53
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(484..508)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      699..708
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(771..789)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ccagaacatt tctctatcga taggtaccac aagtttgtac aaaaaagcag gcttcgtttt
       61 caaaaaacac ctgcgcctgt caacactctg taatcaaaaa cacctgcgcc aaattaaaac
      121 accaaattga atttgtggaa ggcctaggcc actggttaga acaattgaat atggagacgg
      181 aaaaatgcat gaggcatcat cctaaattga ctaccaattt tatataatga gatgggcgac
      241 actcattcca gcagatagcg aataccaacg gatgcaattg ctcaagtcgg gacaattact
      301 gaatccgaca aaaacacctg cgccagagat ttatcagcat ctcgaaaatt acactcgatg
      361 tacacaccaa ttgctcaatt gggagtcttt agtcattccg cactcgacag ataagctttc
      421 gctactgctg ctaataaaat ttcgccaata atgcaaatca gatcttatcg caggccaact
      481 gggacccagc tttcttgtac aaagtggtag atctgcgatc gagctcgccc ggggatcgag
      541 cgcagcggta taaaagggcg cggggtggct gagagcatca gttgtgaatg aatgttcgag
      601 ccgagcagac gtgccgctgc cttcgttaat atcctttgaa taagccaact ttgaatcaca
      661 agacgcatac caaacggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa
      721 acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac
      781 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
      841 atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag
      901 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
      961 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
     1021 acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg
     1081 tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca
     1141 tccaaaaaat tattatcatg gattctaaa
//
