LOCUS       sequence-129346-        1144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_881_Nhe2_EcR-to-HLH4C
FEATURES             Location/Qualifiers
     source          1..1144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    239..263
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(694..718)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    732..823
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(764..783)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    837..885
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc atggtggctt taccaacagt
       61 accggaatgc cgtttggtat gcgtcttgtg attcaaagtt ggcttattca aaggatatta
      121 acgaaggcag cggcacgtct gctcggctcg aacattcatt cacaactgat gctctcagcc
      181 accccgcgcc cttttatacc gctgcgctcg atccccgggc gagctcgatc gcagatctac
      241 cactttgtac aagaaagctg ggtcccagtt ggcctgcgat aagatctgat ttgcattatt
      301 ggcgaaattt tattagcagc agtagcgaaa gcttatctgt cgagtgcgga atgactaaag
      361 actcccaatt gagcaattgg tgtgtacatc gagtgtaatt ttcgagatgc tgataaatct
      421 ctggcgcagg tgtttttgtc ggattcagta attgtcccga cttgagcaat tgcatccgtt
      481 ggtattcgct atctgctgga atgagtgtcg cccatctcat tatataaaat tggtagtcaa
      541 tttaggatga tgcctcatgc atttttccgt ctccatattc aattgttcta accagtggcc
      601 taggccttcc acaaattcaa tttggtgttt taatttggcg caggtgtttt tgattacaga
      661 gtgttgacag gcgcaggtgt tttttgaaaa cgaagcctgc ttttttgtac aaacttgtgg
      721 tacctatcga tagagaaatg ttctggcacc tgcacttgca ctggggacag cctattttgc
      781 tagtttgttt tgtttcgttt tgttttgatg gagagcgtat gttagtacta tcgattcaca
      841 caaaaaacca acacacagat gtaatgaaaa taaagatatt ttattgcggc cgctccaagt
      901 acctcccgta ccttaatatt acttacttat catggtagct tgggctggcg taatagcgaa
      961 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcaaatt
     1021 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     1081 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     1141 ttga
//
