LOCUS       sequence-129351-         426 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  polycistronic cloning vector for pST44 plasmid suite.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST44
FEATURES             Location/Qualifiers
     source          1..426
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(113..131)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      127..174
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        241..332
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcgata tcggtaccag cggataacaa tttcacatcc ggattcgcga acgcgtctcg
       61 agagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
      121 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
      181 ggaggaacta tatccgacta gtagcccgcc taatgagcgg gctttttttt aattccccta
      241 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      301 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      361 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctcgtga
      421 aagtaa
//
