LOCUS       sequence-129353-        1180 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_716_S2-1_GATA-to-DFD
FEATURES             Location/Qualifiers
     source          1..1180
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..52
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(537..561)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      752..761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(824..842)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 cagaacattt ctctatcgat aggtaccaca agtttgtaca aaaaagcagg ctccgaattc
       61 gccctttgca caatttctgt gaagtgacag ccactgagaa atagtgtcaa gtagctctcg
      121 tccgctaata tcaatttaga cgatatacat acggccagat ctggcacgca gtctgcattc
      181 ccaatgatgc gggatggatt tggattgtgg ggcggaatct ctaagttcat taaggcggag
      241 aattacttcc gcgacttggc tccgttttgt taaatatact atgtgtgtat gtgcgtagta
      301 cagcgacttg tacggagtac ataaacatat gcatatctgc ggtttagttg tcgtcattaa
      361 gttgttcgtc gtttgaaaaa tgtgtgtagt tgttggggta actgcgcaca attgccgggc
      421 tacagggtgc tttgagtctt tgtttttgga tttcgttttc gatgtttagc tacgaaagcc
      481 aagcgttgtg ctattttcga attttgtatt ttataacccg ggcaagggcg aattcgaccc
      541 agctttcttg tacaaagtgg tagatctgcg atcgagctcg cccggggatc gagcgcagcg
      601 gtataaaagg gcgcggggtg gctgagagca tcagttgtga atgaatgttc gagccgagca
      661 gacgtgccgc tgccttcgtt aatatccttt gaataagcca actttgaatc acaagacgca
      721 taccaaacgg cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa
      781 gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa
      841 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
      901 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa
      961 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
     1021 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat
     1081 ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt
     1141 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa
//
