LOCUS       sequence-129356-         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single donor or transfer plasmid for source of translation cassette 
            for plasmid suite.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pET3aTr
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             36..58
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(131..150)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(132..150)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(234..253)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tttgctgtcc accagtcatg ctagccatat gtatatctcc ttcttaaagt taaacaaaat
       61 tatttctaga gctagcgaat tccaattgga gctctccgga gccggcacta gagggaaacc
      121 gttgtggtct ccctatagtg agtcgtatta atttcgcggg atcgagatct cgatcctcta
      181 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
      241 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
      301 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
      361 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
      421 aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt
      481 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
      541 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg
      601 ctttcgctgg a
//
