LOCUS       sequence-129359-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  original polycistronic cloning vector for plasmid suite (generation 
            I).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST39
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(82..99)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(82..98)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(155..174)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(156..174)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             411..602
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(596..618)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 atccggatgt gaaattgtta tccgctggta ccgatatcga gctcgactgg gaaaaccctg
       61 gcgaagcttc ccggggaatt cactggccgt cgttttacag gatcttgtac agggcccgga
      121 tccgaggcct tctagaggga aaccgttgtg gtctccctat agtgagtcgt attaatttcg
      181 cgggatcgat gcatggtgcc aacacctaca tctgtattaa cgaagcgctg gcattgaccc
      241 tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt
      301 tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc
      361 atcggtatca ttaccccatg aacagaaatc ccccttacac ggaggcatca gtgaccaaac
      421 aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg
      481 agaaactcaa cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc
      541 acgctgatga gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
      601 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
      661 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgca
//
