LOCUS       sequence-129360-         427 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  original polycistronic cloning vector for plasmid suite (generation 
            I).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST39
FEATURES             Location/Qualifiers
     source          1..427
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(111..129)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      125..172
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        239..330
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gctcgatatc ggtaccagcg gataacaatt tcacatccgg attcgcgaac gcgtctcgag
       61 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      121 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
      181 aggaactata tccgactagt agcccgccta atgagcgggc ttttttttaa ttcccctatt
      241 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      301 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      361 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctcgtgaaa
      421 gtaaaag
//
