LOCUS       sequence-129369-        1127 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_717_S2-1_CGCG-to-DFD
FEATURES             Location/Qualifiers
     source          1..1127
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..52
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(536..560)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      751..760
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(823..841)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 cagaacattt ctctatcgat aggtaccaca agtttgtaca aaaaagcagg ctccgaattc
       61 gccctttgca caatttctgt gaagtgacag ccactgagaa atagtatcaa gtagctctcg
      121 tccgctaata tcaatttaga cgatatacat acggccagat ctggcacgca gtctgcattc
      181 ccaatgatgc gggatggatt tggattgtgg ggcggaatct ctaagtgcga taagatgcgg
      241 agaattatca ttaagtggct ccgttttgtt aaatatacta tgtgtgtatg tgcgtagtac
      301 agcgacttgt acggagtaca taaacatatg catatctgcg gtttagttgt cgacattatc
      361 ttttgttcgt cgtttgaaaa atgtgtgtag ttgttggggt atcattaaga ttgccgggct
      421 acagggtgct ttgagtcttt gtttttggat ttcgttttcg atgtttagct acgaaagcca
      481 agcgttgtgc tattttcgaa ttttgtattt tataacccgg gcaagggcga attcgaccca
      541 gctttcttgt acaaagtggt agatctgcga tcgagctcgc ccggggatcg agcgcagcgg
      601 tataaaaggg cgcggggtgg ctgagagcat cagttgtgaa tgaatgttcg agccgagcag
      661 acgtgccgct gccttcgtta atatcctttg aataagccaa ctttgaatca caagacgcat
      721 accaaacggc attccggtac tgttggtaaa gccaccatgg aagacgccaa aaacataaag
      781 aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag
      841 gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag
      901 gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa
      961 cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc
     1021 tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt
     1081 tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctac
//
