LOCUS       sequence-129370-        1164 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_717_S2-1_CGCG-to-DFD
FEATURES             Location/Qualifiers
     source          1..1164
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    239..263
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(747..771)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    785..876
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(817..836)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    890..938
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc atggtggctt taccaacagt
       61 accggaatgc cgtttggtat gcgtcttgtg attcaaagtt ggcttattca aaggatatta
      121 acgaaggcag cggcacgtct gctcggctcg aacattcatt cacaactgat gctctcagcc
      181 accccgcgcc cttttatacc gctgcgctcg atccccgggc gagctcgatc gcagatctac
      241 cactttgtac aagaaagctg ggtcgaattc gcccttgccc gggttataaa atacaaaatt
      301 cgaaaatagc acaacgcttg gctttcgtag ctaaacatcg aaaacgaaat ccaaaaacaa
      361 agactcaaag caccctgtag cccggcaatc ttaatgatac cccaacaact acacacattt
      421 ttcaaacgac gaacaaaaga taatgtcgac aactaaaccg cagatatgca tatgtttatg
      481 tactccgtac aagtcgctgt actacgcaca tacacacata gtatatttaa caaaacggag
      541 ccacttaatg ataattctcc gcatcttatc gcacttagag attccgcccc acaatccaaa
      601 tccatcccgc atcattggga atgcagactg cgtgccagat ctggccgtat gtatatcgtc
      661 taaattgata ttagcggacg agagctactt gatactattt ctcagtggct gtcacttcac
      721 agaaattgtg caaagggcga attcggagcc tgcttttttg tacaaacttg tggtacctat
      781 cgatagagaa atgttctggc acctgcactt gcactgggga cagcctattt tgctagtttg
      841 ttttgtttcg ttttgttttg atggagagcg tatgttagta ctatcgattc acacaaaaaa
      901 ccaacacaca gatgtaatga aaataaagat attttattgc ggccgctcca agtacctccc
      961 gtaccttaat attacttact tatcatggta gcttgggctg gcgtaatagc gaagaggccc
     1021 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcaa attgtaagcg
     1081 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     1141 aggccgaaat cggcaaaatc cctt
//
