LOCUS       sequence-129376-        1171 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_719_S2-1_GATA-to-ETS21C
FEATURES             Location/Qualifiers
     source          1..1171
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..52
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(539..563)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      754..763
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(826..844)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 cagaacattt ctctatcgat aggtaccaca agtttgtaca aaaaagcagg ctccgaattc
       61 gccctttgca caatttctgt gaagtgacag ccactgagaa atagtgtcaa gtagctctcg
      121 tccgctaata tcaatttaga cgatatacat acggccagat ctggcacgca gtctgcattc
      181 ccaatgatgc gggatggatt tggattgtgg ggcggaatct ctaagtaccg gaaatgcgga
      241 gaattacttc cgcgacttgg ctccgttttg ttaaatatac tatgtgtgta tgtgcgtagt
      301 acagcgactt gtacggagta cataaacata tgcatatctg cggtttagtt gtcgaccgga
      361 aatttgttcg tcgtttgaaa aatgtgtgta gttgttgggg taactgcgca caattgccgg
      421 gctacagggt gctttgagtc tttgtttttg gatttcgttt tcgatgttta gctacgaaag
      481 ccaagcgttg tgctattttc gaattttgta ttttataacc cgggcaaggg cgaattcgac
      541 ccagctttct tgtacaaagt ggtagatctg cgatcgagct cgcccgggga tcgagcgcag
      601 cggtataaaa gggcgcgggg tggctgagag catcagttgt gaatgaatgt tcgagccgag
      661 cagacgtgcc gctgccttcg ttaatatcct ttgaataagc caactttgaa tcacaagacg
      721 cataccaaac ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata
      781 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat
      841 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
      901 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg
      961 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
     1021 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
     1081 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc
     1141 gtttccaaaa aggggttgca aaaaattttg a
//
