LOCUS       sequence-129377-        1197 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_719_S2-1_GATA-to-ETS21C
FEATURES             Location/Qualifiers
     source          1..1197
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    239..263
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(750..774)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    788..879
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(820..839)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    893..941
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc atggtggctt taccaacagt
       61 accggaatgc cgtttggtat gcgtcttgtg attcaaagtt ggcttattca aaggatatta
      121 acgaaggcag cggcacgtct gctcggctcg aacattcatt cacaactgat gctctcagcc
      181 accccgcgcc cttttatacc gctgcgctcg atccccgggc gagctcgatc gcagatctac
      241 cactttgtac aagaaagctg ggtcgaattc gcccttgccc gggttataaa atacaaaatt
      301 cgaaaatagc acaacgcttg gctttcgtag ctaaacatcg aaaacgaaat ccaaaaacaa
      361 agactcaaag caccctgtag cccggcaatt gtgcgcagtt accccaacaa ctacacacat
      421 ttttcaaacg acgaacaaat ttccggtcga caactaaacc gcagatatgc atatgtttat
      481 gtactccgta caagtcgctg tactacgcac atacacacat agtatattta acaaaacgga
      541 gccaagtcgc ggaagtaatt ctccgcattt ccggtactta gagattccgc cccacaatcc
      601 aaatccatcc cgcatcattg ggaatgcaga ctgcgtgcca gatctggccg tatgtatatc
      661 gtctaaattg atattagcgg acgagagcta cttgacacta tttctcagtg gctgtcactt
      721 cacagaaatt gtgcaaaggg cgaattcgga gcctgctttt ttgtacaaac ttgtggtacc
      781 tatcgataga gaaatgttct ggcacctgca cttgcactgg ggacagccta ttttgctagt
      841 ttgttttgtt tcgttttgtt ttgatggaga gcgtatgtta gtactatcga ttcacacaaa
      901 aaaccaacac acagatgtaa tgaaaataaa gatattttat tgcggccgct ccaagtacct
      961 cccgtacctt aatattactt acttatcatg gtagcttggg ctggcgtaat agcgaagagg
     1021 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg caaattgtaa
     1081 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     1141 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggt
//
