LOCUS       sequence-12938-e       16811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16811)
  AUTHORS   Nodine MD, Bartel DP
  TITLE     MicroRNAs prevent precocious gene expression and enable pattern 
            formation during plant embryogenesis.
  JOURNAL   Genes Dev. 2010 Dec 1. 24(23):2678-92.
  PUBMED    21123653
REFERENCE   2  (bases 1 to 16811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 Dec 1. 24(23):2678-92."
COMMENT     Sequence Label: pSPL10p::SPL10-GFP
FEATURES             Location/Qualifiers
     source          1..16811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             37..747
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(64..92)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     687..707
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      773..1025
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1034..1051)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1034..1050)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1043..1065)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1190..1209)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1400..1421
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    1668..1692
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(3288..4436)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(4735..5529)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  5721..6488
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      7111..7822
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(7949..8320)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            8353..8462
                     /note="incP origin of transfer"
     primer_bind     8685..8704
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8938..8955
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(9143..9793)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    10258..10282
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(10443..10643)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     primer_bind     complement(10685..10701)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10685..10701)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10698..10720)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10709..10725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10733..10739,10740..10757,10758..10763))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10778..10799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(11348..11600)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11990..12784)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12101..12120)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     12711..12730
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(12805..12988)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(12841..13020)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    13460..13484
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(16801..16811,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgctcga gatggtagat ctgactagta aaggagaaga acttttcact
       61 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      121 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
      181 actggaaaac tacctgttcc gtggccaaca cttgtcacta ctttctctta tggtgttcaa
      241 tgcttttcaa gatacccaga tcatatgaag cggcacgact tcttcaagag cgccatgcct
      301 gagggatacg tgcaggagag gaccatcttc ttcaaggacg acgggaacta caagacacgt
      361 gctgaagtca agtttgaggg agacaccctc gtcaacagga tcgagcttaa gggaatcgat
      421 ttcaaggagg acggaaacat cctcggccac aagttggaat acaactacaa ctcccacaac
      481 gtatacatca tggccgacaa gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac
      541 aacatcgaag acggcggcgt gcaactcgct gatcattatc aacaaaatac tccaattggc
      601 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
      661 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
      721 acacatggca tggatgaact atacaaagct agctgagagc tcgaatttcc ccgatcgttc
      781 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
      841 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
      901 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
      961 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     1021 agatcgggaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     1081 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     1141 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgcccgc tcctttcgct
     1201 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     1261 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     1321 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     1381 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     1441 tcgggctatt cttttgattt ataagggatt ttgccgattt cggaaccacc atcaaacagg
     1501 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
     1561 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accccagtac
     1621 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
     1681 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
     1741 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
     1801 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
     1861 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
     1921 cctgtgatca aatatcatct ccctcgcaga gatccgaatt atcagccttc ttattcattt
     1981 ctcgcttaac cgtgacaggc tgtcgatctt gagaactatg ccgacataat aggaaatcgc
     2041 tggataaagc cgctgaggaa gctgagtggc gctatttctt tagaagtgaa cgttgacgat
     2101 atcaactccc ctatccattg ctcaccgaat ggtacaggtc ggggacccga agttccgact
     2161 gtcggcctga tgcatccccg gctgatcgac cccagatctg gggctgagaa agcccagtaa
     2221 ggaaacaact gtaggttcga gtcgcgagat cccccggaac caaaggaagt aggttaaacc
     2281 cgctccgatc aggccgagcc acgccaggcc gagaacattg gttcctgtag gcatcgggat
     2341 tggcggatca aacactaaag ctactggaac gagcagaagt cctccggccg ccagttgcca
     2401 ggcggtaaag gtgagcagag gcacgggagg ttgccacttg cgggtcagca cggttccgaa
     2461 cgccatggaa accgcccccg ccaggcccgc tgcgacgccg acaggatcta gcgctgcgtt
     2521 tggtgtcaac accaacagcg ccacgcccgc agttccgcaa atagccccca ggaccgccat
     2581 caatcgtatc gggctaccta gcagagcggc agagatgaac acgaccatca gcggctgcac
     2641 agcgcctacc gtcgccgcga ccccgcccgg caggcggtag accgaaataa acaacaagct
     2701 ccagaatagc gaaatattaa gtgcgccgag gatgaagatg cgcatccacc agattcccgt
     2761 tggaatctgt cggacgatca tcacgagcaa taaacccgcc ggcaacgccc gcagcagcat
     2821 accggcgacc cctcggcctc gctgttcggg ctccacgaaa acgccggaca gatgcgcctt
     2881 gtgagcgtcc ttggggccgt cctcctgttt gaagaccgac agcccaatga tctcgccgtc
     2941 gatgtaggcg ccgaatgcca cggcatctcg caaccgttca gcgaacgcct ccatgggctt
     3001 tttctcctcg tgctcgtaaa cggacccgaa catctctgga gctttcttca gggccgacaa
     3061 tcggatctcg cggaaatcct gcacgtcggc cgctccaagc cgtcgaatct gagccttaat
     3121 cacaattgtc aattttaatc ctctgtttat cggcagttcg tagagcgcgc cgtgcgtccc
     3181 gagcgatact gagcgaagca agtgcgtcga gcagtgcccg cttgttcctg aaatgccagt
     3241 aaagcgctgg ctgctgaacc cccagccgga actgacccca caaggcccta gcgtttgcaa
     3301 tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg
     3361 caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga tccgcacatg
     3421 aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa atagtcgaac
     3481 atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt cgtgtagtgg
     3541 tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg ggacgttttc
     3601 ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag gtgcccaacg
     3661 cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc
     3721 gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta gaacgtgaag
     3781 gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg ccacaccagt
     3841 tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg
     3901 tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac
     3961 agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca cggcgcaata
     4021 tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc
     4081 tgcttggcct cgctgacctg ttttgccagg tcctcgccgg cggtttttcg cttcttggtc
     4141 gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc ctcctgttcg
     4201 agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg agccagttgc
     4261 acgctgtcgc gctcgatctt ggccgtagct tgctggacca tcgagccgac ggactggaag
     4321 gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa
     4381 aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg attcattcac
     4441 cctccttgcg ggattgcccc gactcacgcc ggggcaatgt gcccttattc ctgatttgac
     4501 ccgcctggtg ccttggtgtc cagataatcc accttatcgg caatgaagtc ggtcccgtag
     4561 accgtctggc cgtccttctc gtacttggta ttccgaatct tgccctgcac gaataccagc
     4621 gaccccttgc ccaaatactt gccgtgggcc tcggcctgag agccaaaaca cttgatgcgg
     4681 aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta ggtactaaaa
     4741 caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa
     4801 gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca
     4861 gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact
     4921 tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag
     4981 ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg
     5041 agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc
     5101 caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga
     5161 gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc
     5221 ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc
     5281 atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat
     5341 catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt
     5401 taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc
     5461 cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat
     5521 tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct
     5581 aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag
     5641 aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg
     5701 attttgaaac cacaattatg ggtgatgctg ccaacttact gatttagtgt atgatggtgt
     5761 ttttgaggtg ctccagtggc ttctgtgtct atcagctgtc cctcctgttc agctactgac
     5821 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ctctcactgc
     5881 cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa
     5941 atcattgata tggccatgaa tggcgttgga tgccgggcaa cagcccgcat tatgggcgtt
     6001 ggcctcaaca cgattttacg tcacttaaaa aactcaggcc gcagtcggta acctcgcgca
     6061 tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggctatgtcg
     6121 gggctaaatc gcgccagcgc tggctgtttt acgcgtatga cagtctccgg aagacggttg
     6181 ttgcgcacgt attcggtgaa cgcactatgg cgacgctggg gcgtcttatg agcctgctgt
     6241 caccctttga cgtggtgata tggatgacgg atggctggcc gctgtatgaa tcccgcctga
     6301 agggaaagct gcacgtaatc agcaagcgat atacgcagcg aattgagcgg cataacctga
     6361 atctgaggca gcacctggca cggctgggac ggaagtcgct gtcgttctca aaatcggtgg
     6421 agctgcatga caaagtcatc gggcattatc tgaacataaa acactatcaa taagttggag
     6481 tcattaccca attatgatag aatttacaag ctataaggtt attgtcctgg gtttcaagca
     6541 ttagtccatg caagttttta tgctttgccc attctataga tatattgata agcgcgctgc
     6601 ctatgccttg ccccctgaaa tccttacata cggcgatatc ttctatataa aagatatatt
     6661 atcttatcag tattgtcaat atattcaagg caatctgcct cctcatcctc ttcatcctct
     6721 tcgtcttggt agctttttaa atatggcgct tcatagagta attctgtaaa ggtccaattc
     6781 tcgttttcat acctcggtat aatcttacct atcacctcaa atggttcgct gggtttatcg
     6841 cacccccgaa cacgagcacg gcacccgcga ccactatgcc aagaatgccc aaggtaaaaa
     6901 ttgccggccc cgccatgaag tccgtgaatg ccccgacggc cgaagtgaag ggcaggccgc
     6961 cacccaggcc gccgccctca ctgcccggca cctggtcgct gaatgtcgat gccagcacct
     7021 gcggcacgtc aatgcttccg ggcgtcgcgc tcgggctgat cgcccatccc gttactgccc
     7081 cgatcccggc aatggcaagg actgccagcg ctgccatttt tggggtgagg ccgttcgcgg
     7141 ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga
     7201 gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta
     7261 aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg
     7321 aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat
     7381 gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc
     7441 cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg
     7501 cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag
     7561 gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg
     7621 ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag
     7681 ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc
     7741 gtttctggcg cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc
     7801 ccggtgagcg tcgcaaaggc gctcggtctt gccttgctcg tcggtgatgt acttcaccag
     7861 ctccgcgaag tcgctcttct tgatggagcg catggggacg tgcttggcaa tcacgcgcac
     7921 cccccggccg ttttagcggc taaaaaagtc atggctctgc cctcgggcgg accacgccca
     7981 tcatgacctt gccaagctcg tcctgcttct cttcgatctt cgccagcagg gcgaggatcg
     8041 tggcatcacc gaaccgcgcc gtgcgcgggt cgtcggtgag ccagagtttc agcaggccgc
     8101 ccaggcggcc caggtcgcca ttgatgcggg ccagctcgcg gacgtgctca tagtccacga
     8161 cgcccgtgat tttgtagccc tggccgacgg ccagcaggta ggccgacagg ctcatgccgg
     8221 ccgccgccgc cttttcctca atcgctcttc gttcgtctgg aaggcagtac accttgatag
     8281 gtgggctgcc cttcctggtt ggcttggttt catcagccat ccgcttgccc tcatctgtta
     8341 cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg
     8401 aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc
     8461 ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg
     8521 tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg
     8581 gttatgcagc ggaaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     8641 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     8701 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     8761 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     8821 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     8881 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     8941 gagtcagtga gcgaggaagc ggaagagcgc cagaaggccg ccagagaggc cgagcgcggc
     9001 cgtgaggctt ggacgctagg gcagggcatg aaaaagcccg tagcgggctg ctacgggcgt
     9061 ctgacgcggt ggaaaggggg aggggatgtt gtctacatgg ctctgctgta gtgagtgggt
     9121 tgcgctccgg cagcggtcct gatcaatcgt caccctttct cggtccttca acgttcctga
     9181 caacgagcct ccttttcgcc aatccatcga caatcaccgc gagtccctgc tcgaacgctg
     9241 cgtccggacc ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc gagagcggag
     9301 cctgttcaac ggtgccgccg cgctcgccgg catcgctgtc gccggcctgc tcctcaagca
     9361 cggccccaac agtgaagtag ctgattgtca tcagcgcatt gacggcgtcc ccggccgaaa
     9421 aacccgcctc gcagaggaag cgaagctgcg cgtcggccgt ttccatctgc ggtgcgcccg
     9481 gtcgcgtgcc ggcatggatg cgcgcgccat cgcggtaggc gagcagcgcc tgcctgaagc
     9541 tgcgggcatt cccgatcaga aatgagcgcc agtcgtcgtc ggctctcggc accgaatgcg
     9601 tatgattctc cgccagcatg gcttcggcca gtgcgtcgag cagcgcccgc ttgttcctga
     9661 agtgccagta aagcgccggc tgctgaaccc ccaaccgttc cgccagtttg cgtgtcgtca
     9721 gaccgtctac gccgacctcg ttcaacaggt ccagggcggc acggatcact gtattcggct
     9781 gcaactttgt catgcttgac actttatcac tgataaacat aatatgtcca ccaacttatc
     9841 agtgataaag aatccgcgcg ttcaatcgga ccagcggagg ctggtccgga ggccagacgt
     9901 gaaacccaac atacccctga tcgtaattct gagcactgtc gcgctcgacg ctgtcggcat
     9961 cggcctgatt atgccggtgc tgccgggcct cctgcgcgat ctggttcact cgaacgacgt
    10021 caccgcccac tatggcattc tgctggcgct gtatgcgttg gtgcaatttg cctgcgcacc
    10081 tgtgctgggc gcgctgtcgg atcgtttcgg gcggcggcca atcttgctcg tctcgctggc
    10141 cggcgccaga tctggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
    10201 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt
    10261 tacccgccaa tatatcctgt caaacactga tagtttaaac tgaaggcggg aaacgacaat
    10321 ctgatcatga gcggagaatt aagggagtca cgttatgacc cccgccgatg acgcgggaca
    10381 agccgtttta cgtttggaac tgacagaacc gcaacgttga aggagccact cagccaattc
    10441 ccatcttgaa agaaatatag tttaaatatt tattgataaa ataacaagtc aggtattata
    10501 gtccaagcaa aaacataaat ttattgatgc aagtttaaat tcagaaatat ttcaataact
    10561 gattatatca gctggtacat tgccgtagat gaaagactga gtgcgatatt atgtgtaata
    10621 cataaattga tgatatagct agcttagctc atcgggggat cctctagagc ttggcgtaat
    10681 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
    10741 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    10801 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    10861 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gccaaagaca aaagggcgac
    10921 attcaaccga ttgagggagg gaaggtaaat attgacggaa attattcatt aaaggtgaat
    10981 tatcaccgtc accgacttga gccatttggg aattagagcc agcaaaatca ccagtagcac
    11041 cattaccatt agcaaggccg gaaacgtcac caatgaaacc atcgatagca gcaccgtaat
    11101 cagtagcgac agaatcaagt ttgcctttag cgtcagactg tagcgcgttt tcatcggcat
    11161 tttcggtcat agccccctta ttagcgtttg ccatcttttc ataatcaaaa tcaccggaac
    11221 cagagccacc accggaaccg cctccctcag agccgccacc ctcagaaccg ccaccctcag
    11281 agccaccacc ctcagagccg ccaccagaac caccaccaga gccgccgcca gcattgacag
    11341 gaggcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
    11401 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
    11461 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
    11521 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
    11581 tgccaaatgt ttgaacgatc ggggatcatc cgggtctgtg gcgggaactc cacgaaaata
    11641 tccgaacgca gcaagatatc gcggtgcatc tcggtcttgc ctgggcagtc gccgccgacg
    11701 ccgttgatgt ggacgccggg cccgatcata ttgtcgctca ggatcgtggc gttgtgcttg
    11761 tcggccgttg ctgtcgtaat gatatcggca ccttcgaccg cctgttccgc agagatcccg
    11821 tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg
    11881 aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat
    11941 ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct cagaagaact
    12001 cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca
    12061 cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg
    12121 ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc
    12181 ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcat
    12241 cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat
    12301 gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
    12361 cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc
    12421 gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga
    12481 gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt
    12541 cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt
    12601 cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct
    12661 gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat
    12721 agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa
    12781 tcatgcgaaa cgatccagat ccggtgcaga ttatttggat tgagagtgaa tatgagactc
    12841 taattggata ccgaggggaa tttatggaac gtcagtggag catttttgac aagaaatatt
    12901 tgctagctga tagtgacctt aggcgacttt tgaacgcgca ataatggttt ctgacgtatg
    12961 tgcttagctc attaaactcc agaaacccgc ggctgagtgg ctccttcaac gttgcggttc
    13021 tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact
    13081 cccttaattc tccgctcatg atcttgatcc cctgcgccat cagatccttg gcggcaagaa
    13141 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa
    13201 ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact
    13261 gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct
    13321 gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct
    13381 ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgaa gcttgcatgc ctgcaggtcg
    13441 actctagagc ccgggtacca caagtttgta caaaaaagca ggctccgcgg ccgccccctt
    13501 cacctcatca tctttcacga actcgttacc gttttactta aatgaaataa ttcttagatt
    13561 tttttttccg ttcttgtaga ttttctaggt aaaaaaataa tctcatttgc tctagatatg
    13621 ttacaaaaca ttttctatca aatcattttt ctatctacaa gaagaagaag aagaaaaata
    13681 ctaattaata aatgaatatt tgtctactat tagaaaactt ttatctgcag taaactactt
    13741 caaaattatt tgatgtcatg taatctactt caaaatttac gccaaaagaa cacgaaattt
    13801 acatgcgctg taacaaaaaa gttacccaat catgaaagca agaatcgaag agaaaaatta
    13861 tttaaaaagc aaaaagaaaa cagtaagaaa aaggaaaggg aataatggaa caaaaatgaa
    13921 aaaaaattgg aaggatgaaa ttaatcgaaa gcagctcatc cgattcccct gaggcgatta
    13981 tggtgtctcc tccttctcac tctcgctctt agtagtagtc tttcttctcc gatcgccgac
    14041 taacctccgg agctttcttc ggcggtctct cctctctgtt ttctacttga ggtttctctc
    14101 ttctcttact ttttttattc tttgtgcata tctctcttct cttactttac tcaccaaatt
    14161 tctaggaata ctttttctcc tgagatttgg gcttttaatt tagcttaatt cttctgttaa
    14221 gtagaaatat atcatggcga atctatgaaa ttatttcttc tgttaagtag acattgaaat
    14281 ttgttacaat ggcgaatcgt gttttttttt tggtctgtct ccattttttg gtattgtctc
    14341 taagaaaata agacgtttgt ggtatttagt ttattaggcg aattggattt gttgaagcat
    14401 ggataacttg tatcttaatc ccttggggtt agttagatga attgaatggt cgagggacca
    14461 ttttgctttt tcatgggaaa ccctggctga ggggaccgtg attgggatcg aaacctaact
    14521 tgctgtctca ttttgttagt tcaatcttta ttgctggaga tagatccctt tcattggctt
    14581 agcttggtgc cgttttcttt gacatatact taattcaata tgtgtgtatc tgtagactca
    14641 atagtaagct ttagattaac cctttatttc atttaaacct tagctaagaa ctttctgcaa
    14701 gtacaaagaa ggaatgggta gggaagggtt tgcaattatg atggctatag ggttttaatt
    14761 gacttgttga agtcgtgttc tttaacaagt tgttgatttt ctgggcaatc tgatttgagg
    14821 agtgtgggac cagttggaat aaatgccact tagtttttgt gtgatggaga gcacaagagg
    14881 gagctaagaa aagtaatacc ctttctcttg ccctttttgt tggtctgggt gaaacatagc
    14941 aaggtttctc ttgctgaggt tattgataaa cgggtgagtt aaataaaaac gttgatcagc
    15001 agtgtgtggt gcttggaatt cataaggttt ttcttctttc ttatgctttt ggtagttaat
    15061 ggtattgtct attaggtatc tgtttgggtt gccagcgtta tttataggct tgacaagttt
    15121 tctcttattt ttgatgtttg gctttcattt acaatcacta gttctgcaaa cttatggttg
    15181 tttcagtttt tgttactctg gcttttggtg ataggtctat gaaatcaacc cacatcttga
    15241 atggactgca acatggtatc ttcgttcccg tgggactggg agaatttgat catgtccaat
    15301 cagtcgaaga ctgaaaatga aaaaaaacag caatctactg agtgggaatt tgaaaaaggt
    15361 gaaggaattg aatctatagt tccagatttc ttaggctttg agaaagtcag tagtggctct
    15421 gctactagtt tctggcacac tgccgtatca aaaagctcgc agtcgacctc tatcaactca
    15481 tcatctcccg aggacaaacg atgcaatctt gcatcacaaa gttcccctgg agattcttcc
    15541 agcaacatag attttctcca ggtgaaacca tccacagctc tcgaggtacc tattgcctca
    15601 gctgaatcag atctttgttt gaaactagga aagcggacat actctgaaga attttggggt
    15661 aggaacaata atgacctttc agcggtttct atgaatttgt tgactccatc tgttgttgct
    15721 cggaagaaaa ccaaatcgtg tggtcagagc atgcaagttc cgcgttgcca aattgatggc
    15781 tgtgagctgg atctctcatc ttctaaggat tatcatcgca agcatagagt ctgcgaaacg
    15841 cattcaaagt gcccaaaagt tgttgtgagt ggcctggaac gtcgtttctg ccaacagtgt
    15901 agcaggtaaa ccatgattat tgttctgact gcattctctt tactgcaaaa tgctagttct
    15961 gtttagtcgt ccagcaataa ctttaacatg ccctaatgtt atggtagaga attgatttct
    16021 tttctaatgt tcaggttcca tgctgtctca gaatttgatg aaaagaaacg aagctgccgc
    16081 aaacgtcttt ctcatcataa tgcaaggcgt cgcaagccac aaggagtatt tccactgaat
    16141 tcagagaggg tgttcggtaa gaacctacac tacacaagtt gatattagca atgtctcttc
    16201 aggatctttc catctgtcaa ttctctcttt ctctatcaca cacaccaaca acgaattttt
    16261 tgcttttaca gatcgaagac agcatacaag tatgttgtgg aatgggttgt cccttaacac
    16321 gagatctgaa gaaaagtata catggggtac cacttatgag acaaagccta cacagatgga
    16381 aagcggcttt actctgagct tccagagagg taatggctct gaggaccaac tgtttactgg
    16441 tagcaccctc tctttctctg cgtttcaaac atctggtggg ttctcagcag ggaaatccaa
    16501 cattcaactt ccagacaaag gttgatatct tcacttcttt accacatctt gtatttgttg
    16561 tgcatatttg ctgatggatt ggcctttttt agatctctgg ttttgaaact gagtaatatg
    16621 ttttggcagg tgtgggagaa tgctcaggag gcctccatga atctcatgat ttctacagtg
    16681 ctctctctct tctgtcaact acttcggatt cacaagggat caaacacact cccgtggccg
    16741 aaccaccgcc aatatttggc actttcccta gtcatttcat cgcaaagggt gggcgcgccg
    16801 acccagcttt c
//
