LOCUS       sequence-129385-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-His tagged nonglycosylated human serum transferrin 
            unable to bind iron in the C-lobe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Mason AB, Halbrooks PJ, Larouche JR, Briggs SK, Moffett ML, Ramsey 
            JE, Connolly SA, Smith VC, MacGillivray RT
  TITLE     Expression, purification, and characterization of authentic 
            monoferric and apo-human serum transferrins.
  JOURNAL   Protein Expr Purif. 2004 Aug;36(2):318-26.
  PUBMED    15249056
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2004-08"; volume: "36(2)"; pages: "318-26"
COMMENT     Sequence Label: pNUTN6His Y426F/Y517F hTFNG
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             107..124
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             125..136
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agcgtcaccc ctagagtcga cgcggccgcc cgggaagatg aggctcgccg tgggagccct
       61 gctggtctgc gccgtcctag ggctgtgtct ggctgtccct gataaacatc atcatcatca
      121 tcatatcgag ggaagggtcc ctgataaaac tgtgagatgg tgtgcagtgt cggagcatga
      181 ggccactaag tgccagagtt tccgcgacca tatgaaaagc gtcattccat ccgatggtcc
      241 cagtgttgct tgtgtgaaga aagcctccta ccttgattgc atcagggcca ttgcggcaaa
      301 cgaagcggat gctgtgacac tggatgcagg tttggtgtat gatgcttacc tggctcccaa
      361 taacctgaag cctgtggtgg cagagttcta tgggtcaaaa gaggatccac agactttcta
      421 ttatgctgtt gctgtggtga agaaggatag tggcttccag atgaaccagc ttcgaggcaa
      481 gaagtcctgc cacacgggtc taggcaggtc cgctgggtgg aacatcccca taggcttact
      541 ttactgtgac ttacctgagc cacgtaaacc tcttgagaaa gcagtggcca atttcttctc
      601 gggcagctgt gccccttgtg cggatgggac ggacttcccc cagctgtgtc aactgtgtcc
      661 agggtgtggc tgctccaccc ttaaccaata cttcggctac tcgggagcct tcaagtgtct
      721 gaaggatggt gctggggatg tggcctttgt caagcactcg actatatttg agaacttggc
      781 aaacaaggct gacagggacc agtatgagct gctttgcctg gacaacaccc ggaagccggt
      841 agatgaatac aaggactgcc acttggccca ggtcccttct cataccgtcg tggcccgaag
      901 tat
//
