LOCUS       sequence-129387-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-His tagged nonglycosylated human serum transferrin 
            unable to bind iron in either the N-lobe or the C-lobeN-lobe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Mason AB, Halbrooks PJ, Larouche JR, Briggs SK, Moffett ML, Ramsey 
            JE, Connolly SA, Smith VC, MacGillivray RT
  TITLE     Expression, purification, and characterization of authentic 
            monoferric and apo-human serum transferrins.
  JOURNAL   Protein Expr Purif. 2004 Aug;36(2):318-26.
  PUBMED    15249056
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2004-08"; volume: "36(2)"; pages: "318-26"
COMMENT     Sequence Label: pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             106..123
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             124..135
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gcgtcacccc tagagtcgac gcggccgccc gggaagatga ggctcgccgt gggagccctg
       61 ctggtctgcg ccgtcctagg gctgtgtctg gctgtccctg ataaacatca tcatcatcat
      121 catatcgagg gaagggtccc tgataaaact gtgagatggt gtgcagtgtc ggagcatgag
      181 gccactaagt gccagagttt ccgcgaccat atgaaaagcg tcattccatc cgatggtccc
      241 agtgttgctt gtgtgaagaa agcctcctac cttgattgca tcagggccat tgcggcaaac
      301 gaagcggatg ctgtgacact ggatgcaggt ttggtgtatg atgcttacct ggctcccaat
      361 aacctgaagc ctgtggtggc agagttctat gggtcaaaag aggatccaca gactttcttt
      421 tatgctgttg ctgtggtgaa gaaggatagt ggcttccaga tgaaccagct tcgaggcaag
      481 aagtcctgcc acacgggtct aggcaggtcc gctgggtgga acatccccat aggcttactt
      541 tactgtgact tacctgagcc acgtaaacct cttgagaaag cagtggccaa tttcttctcg
      601 ggcagctgtg ccccttgtgc ggatgggacg gacttccccc agctgtgtca actgtgtcca
      661 gggtgtggct gctccaccct taaccaatac ttcggcttct cgggagcctt caagtgtctg
      721 aaggatggtg ctggggatgt ggcctttgtc aagcactcga ctatatttga gaacttggca
      781 aacaaggctg acagggacca gtatgagctg ctttgcctgg acaacacccg gaagccggta
      841 gatgaataca aggactgc
//
