LOCUS       sequence-12939-e       16259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16259)
  AUTHORS   Nodine MD, Bartel DP
  TITLE     MicroRNAs prevent precocious gene expression and enable pattern 
            formation during plant embryogenesis.
  JOURNAL   Genes Dev. 2010 Dec 1. 24(23):2678-92.
  PUBMED    21123653
REFERENCE   2  (bases 1 to 16259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 Dec 1. 24(23):2678-92."
COMMENT     Sequence Label: pSPL10F13R13::pBIB-BAS (gSPL10)
FEATURES             Location/Qualifiers
     source          1..16259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      31..283
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(292..309)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(292..308)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(301..323)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(448..467)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     658..679
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    926..950
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(2546..3694)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(3993..4787)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  4979..5746
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      6369..7080
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(7207..7578)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            7611..7720
                     /note="incP origin of transfer"
     primer_bind     7943..7962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8196..8213
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(8401..9051)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    9516..9540
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(9701..9901)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             complement(9924..10475)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEM"
     misc_feature    10477..10531
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(10573..10953)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    11293..11317
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(16249..16259,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
       61 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
      121 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
      181 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
      241 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt cactggccgt
      301 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
      361 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
      421 acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      481 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      541 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
      601 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      661 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
      721 aagggatttt gccgatttcg gaaccaccat caaacaggat tttcgcctgc tggggcaaac
      781 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      841 gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt
      901 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc
      961 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag
     1021 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
     1081 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
     1141 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc
     1201 ctcgcagaga tccgaattat cagccttctt attcatttct cgcttaaccg tgacaggctg
     1261 tcgatcttga gaactatgcc gacataatag gaaatcgctg gataaagccg ctgaggaagc
     1321 tgagtggcgc tatttcttta gaagtgaacg ttgacgatat caactcccct atccattgct
     1381 caccgaatgg tacaggtcgg ggacccgaag ttccgactgt cggcctgatg catccccggc
     1441 tgatcgaccc cagatctggg gctgagaaag cccagtaagg aaacaactgt aggttcgagt
     1501 cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac
     1561 gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct
     1621 actggaacga gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc
     1681 acgggaggtt gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc
     1741 aggcccgctg cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc
     1801 acgcccgcag ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc
     1861 agagcggcag agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc
     1921 ccgcccggca ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt
     1981 gcgccgagga tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc
     2041 acgagcaata aacccgccgg caacgcccgc agcagcatac cggcgacccc tcggcctcgc
     2101 tgttcgggct ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc
     2161 tcctgtttga agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg
     2221 gcatctcgca accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg
     2281 gacccgaaca tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc
     2341 acgtcggccg ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct
     2401 ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag
     2461 tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc
     2521 cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc
     2581 aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc
     2641 gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga
     2701 gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca
     2761 gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga
     2821 tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga
     2881 agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg
     2941 ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg
     3001 accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg
     3061 tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct
     3121 cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca
     3181 gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt
     3241 ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt
     3301 ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt
     3361 ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga
     3421 tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg
     3481 cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg
     3541 ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga
     3601 cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt
     3661 gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga
     3721 ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca
     3781 gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt
     3841 acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc
     3901 cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct
     3961 tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa
     4021 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
     4081 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
     4141 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
     4201 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
     4261 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
     4321 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
     4381 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
     4441 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
     4501 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
     4561 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
     4621 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
     4681 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
     4741 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
     4801 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
     4861 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
     4921 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg
     4981 tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt
     5041 ctgtgtctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa
     5101 gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt
     5161 tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg
     5221 gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc
     5281 acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat
     5341 cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg
     5401 gctgttttac gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg
     5461 cactatggcg acgctggggc gtcttatgag cctgctgtca ccctttgacg tggtgatatg
     5521 gatgacggat ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag
     5581 caagcgatat acgcagcgaa ttgagcggca taacctgaat ctgaggcagc acctggcacg
     5641 gctgggacgg aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg
     5701 gcattatctg aacataaaac actatcaata agttggagtc attacccaat tatgatagaa
     5761 tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg
     5821 ctttgcccat tctatagata tattgataag cgcgctgcct atgccttgcc ccctgaaatc
     5881 cttacatacg gcgatatctt ctatataaaa gatatattat cttatcagta ttgtcaatat
     5941 attcaaggca atctgcctcc tcatcctctt catcctcttc gtcttggtag ctttttaaat
     6001 atggcgcttc atagagtaat tctgtaaagg tccaattctc gttttcatac ctcggtataa
     6061 tcttacctat cacctcaaat ggttcgctgg gtttatcgca cccccgaaca cgagcacggc
     6121 acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc
     6181 cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca cccaggccgc cgccctcact
     6241 gcccggcacc tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg
     6301 cgtcgcgctc gggctgatcg cccatcccgt tactgccccg atcccggcaa tggcaaggac
     6361 tgccagcgct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg
     6421 ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg
     6481 gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg
     6541 gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc
     6601 aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc
     6661 tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc
     6721 ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga
     6781 aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg
     6841 gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag
     6901 tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac
     6961 gccggcggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc
     7021 catagacggc cgccagccca gcggcgaggg caaccagccc ggtgagcgtc gcaaaggcgc
     7081 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
     7141 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
     7201 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
     7261 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
     7321 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
     7381 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
     7441 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
     7501 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
     7561 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
     7621 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
     7681 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
     7741 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
     7801 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
     7861 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7921 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7981 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8041 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8101 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8161 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8221 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     8281 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     8341 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     8401 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     8461 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     8521 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     8581 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     8641 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     8701 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     8761 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     8821 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     8881 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     8941 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     9001 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     9061 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     9121 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     9181 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     9241 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     9301 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     9361 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     9421 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     9481 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     9541 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     9601 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     9661 acagaaccgc aacgttgaag gagccactca gccaattccc atcttgaaag aaatatagtt
     9721 taaatattta ttgataaaat aacaagtcag gtattatagt ccaagcaaaa acataaattt
     9781 attgatgcaa gtttaaattc agaaatattt caataactga ttatatcagc tggtacattg
     9841 ccgtagatga aagactgagt gcgatattat gtgtaataca taaattgatg atatagctag
     9901 cttagctcat cgggggatct tcatcacatc tcggtgacgg gcaggaccgg acggggcggt
     9961 actggcaggc tgaagtccag ctgccagaaa cccacgtcat gccagttccc gtgcttgaag
    10021 ccggccgccc gcagcatgcc gcggggggca tatccgagcg cctcgtgcat gcgcacgctc
    10081 gggtcgttgg gcagcccgat gacagcgacc acgctcttga agccctgtgc ctccagggac
    10141 ttcagcaggt gggtgtagag cgtggagccc agtcccgtcc gctggtggcg gggggagacg
    10201 tacacggttg actcggccgt ccagtcgtag gcgttgcgtg ccttccaggg gcccgcgtag
    10261 gcgatgccgg cgacctcgcc gtccacctcg gcgacgagcc agggatagcg ctcccgcaga
    10321 cggacgaggt cgtccgtcca ctcctgcggt tcctgcggct cggtacggaa gttgaccgtg
    10381 cttgtctcga tgtagtggtt gacgatggtg cagaccgccg gcatgtccgc ctcggtggca
    10441 cggcggatgt cggccgggcg tcgttctggg ctcatggtaa ttgtaaatag taattgtaat
    10501 gttgttgttg tttgttgttg ttggtaattg ttgtaaaaat agagctatta tactcgaata
    10561 attcgctaga gtcgatttgg tgtatcgaga ttggttatga aattcagatg ctagtgtaat
    10621 gtattggtaa tttgggaaga tataatagga agcaaggcta tttatccatt tctgaaaagg
    10681 cgaaatggcg tcaccgcgag cgtcacgcgc attccgttct tgctgtaaag cgttgtttgg
    10741 tacacttttg actagcgagg cttggcgtgt cagcgtatct attcaaaagt cgttaatggc
    10801 tgcggatcaa gaaaaagttg gaatagaaac agaatacccg cgaaattcag gcccggttgc
    10861 catgtcctac acgccgaaat aaacgaccaa attagtagaa aaataaaaac tgactcggat
    10921 acttacgtca cgtcttgcgc actgatttga aaaatctcaa tataaacaaa gacggccaca
    10981 agaaaaaacc aaaacaccga tattcattaa tcttatctag tttctcaaaa aaattcatat
    11041 cttccacacg tgaaaatgcc aatttctcag acctacctcg gctctgcgaa ggcccccgct
    11101 ggtatcaaaa gtttttattt catccgacat ggcgcgaccg acctcaacga gaaggaaatt
    11161 gtcgtgaacg gtgagaagct ctggggcgtg caaggttccg gaacgaacat cggtctcaat
    11221 gcaaaagggg aacgccaggc tctgttggcc caagcttgca tgcctgcagg tcgactctag
    11281 agcccgggta ccacaagttt gtacaaaaaa gcaggctccg cggccgcccc cttcaccttc
    11341 agttttggta agtttttgtt actcatcatc tttcacgaac tcgttaccgt tttacttaaa
    11401 tgaaataatt cttagatttt tttttccgtt cttgtagatt ttctaggtaa aaaaataatc
    11461 tcatttgctc tagatatgtt acaaaacatt ttctatcaaa tcatttttct atctacaaga
    11521 agaagaagaa gaaaaatact aattaataaa tgaatatttg tctactatta gaaaactttt
    11581 atctgcagta aactacttca aaattatttg atgtcatgta atctacttca aaatttacgc
    11641 caaaagaaca cgaaatttac atgcgctgta acaaaaaagt tacccaatca tgaaagcaag
    11701 aatcgaagag aaaaattatt taaaaagcaa aaagaaaaca gtaagaaaaa ggaaagggaa
    11761 taatggaaca aaaatgaaaa aaaattggaa ggatgaaatt aatcgaaagc agctcatccg
    11821 attcccctga ggcgattatg gtgtctcctc cttctcactc tcgctcttag tagtagtctt
    11881 tcttctccga tcgccgacta acctccggag ctttcttcgg cggtctctcc tctctgtttt
    11941 ctacttgagg tttctctctt ctcttacttt ttttattctt tgtgcatatc tctcttctct
    12001 tactttactc accaaatttc taggaatact ttttctcctg agatttgggc ttttaattta
    12061 gcttaattct tctgttaagt agaaatatat catggcgaat ctatgaaatt atttcttctg
    12121 ttaagtagac attgaaattt gttacaatgg cgaatcgtgt tttttttttg gtctgtctcc
    12181 attttttggt attgtctcta agaaaataag acgtttgtgg tatttagttt attaggcgaa
    12241 ttggatttgt tgaagcatgg ataacttgta tcttaatccc ttggggttag ttagatgaat
    12301 tgaatggtcg agggaccatt ttgctttttc atgggaaacc ctggctgagg ggaccgtgat
    12361 tgggatcgaa acctaacttg ctgtctcatt ttgttagttc aatctttatt gctggagata
    12421 gatccctttc attggcttag cttggtgccg ttttctttga catatactta attcaatatg
    12481 tgtgtatctg tagactcaat agtaagcttt agattaaccc tttatttcat ttaaacctta
    12541 gctaagaact ttctgcaagt acaaagaagg aatgggtagg gaagggtttg caattatgat
    12601 ggctataggg ttttaattga cttgttgaag tcgtgttctt taacaagttg ttgattttct
    12661 gggcaatctg atttgaggag tgtgggacca gttggaataa atgccactta gtttttgtgt
    12721 gatggagagc acaagaggga gctaagaaaa gtaataccct ttctcttgcc ctttttgttg
    12781 gtctgggtga aacatagcaa ggtttctctt gctgaggtta ttgataaacg ggtgagttaa
    12841 ataaaaacgt tgatcagcag tgtgtggtgc ttggaattca taaggttttt cttctttctt
    12901 atgcttttgg tagttaatgg tattgtctat taggtatctg tttgggttgc cagcgttatt
    12961 tataggcttg acaagttttc tcttattttt gatgtttggc tttcatttac aatcactagt
    13021 tctgcaaact tatggttgtt tcagtttttg ttactctggc ttttggtgat aggtctatga
    13081 aatcaaccca catcttgaat ggactgcaac atggtatctt cgttcccgtg ggactgggag
    13141 aatttgatca tgtccaatca gtcgaagact gaaaatgaaa aaaaacagca atctactgag
    13201 tgggaatttg aaaaaggtga aggaattgaa tctatagttc cagatttctt aggctttgag
    13261 aaagtcagta gtggctctgc tactagtttc tggcacactg ccgtatcaaa aagctcgcag
    13321 tcgacctcta tcaactcatc atctcccgag gacaaacgat gcaatcttgc atcacaaagt
    13381 tcccctggag attcttccag caacatagat tttctccagg tgaaaccatc cacagctctc
    13441 gaggtaccta ttgcctcagc tgaatcagat ctttgtttga aactaggaaa gcggacatac
    13501 tctgaagaat tttggggtag gaacaataat gacctttcag cggtttctat gaatttgttg
    13561 actccatctg ttgttgctcg gaagaaaacc aaatcgtgtg gtcagagcat gcaagttccg
    13621 cgttgccaaa ttgatggctg tgagctggat ctctcatctt ctaaggatta tcatcgcaag
    13681 catagagtct gcgaaacgca ttcaaagtgc ccaaaagttg ttgtgagtgg cctggaacgt
    13741 cgtttctgcc aacagtgtag caggtaaacc atgattattg ttctgactgc attctcttta
    13801 ctgcaaaatg ctagttctgt ttagtcgtcc agcaataact ttaacatgcc ctaatgttat
    13861 ggtagagaat tgatttcttt tctaatgttc aggttccatg ctgtctcaga atttgatgaa
    13921 aagaaacgaa gctgccgcaa acgtctttct catcataatg caaggcgtcg caagccacaa
    13981 ggagtatttc cactgaattc agagagggtg ttcggtaaga acctacacta cacaagttga
    14041 tattagcaat gtctcttcag gatctttcca tctgtcaatt ctctctttct ctatcacaca
    14101 caccaacaac gaattttttg cttttacaga tcgaagacag catacaagta tgttgtggaa
    14161 tgggttgtcc cttaacacga gatctgaaga aaagtataca tggggtacca cttatgagac
    14221 aaagcctaca cagatggaaa gcggctttac tctgagcttc cagagaggta atggctctga
    14281 ggaccaactg tttactggta gcaccctctc tttctctgcg tttcaaacat ctggtgggtt
    14341 ctcagcaggg aaatccaaca ttcaacttcc agacaaaggt tgatatcttc acttctttac
    14401 cacatcttgt atttgttgtg catatttgct gatggattgg ccttttttag atctctggtt
    14461 ttgaaactga gtaatatgtt ttggcaggtg tgggagaatg ctcaggaggc ctccatgaat
    14521 ctcatgattt ctacagtgct ctctctcttc tgtcaactac ttcggattca caagggatca
    14581 aacacactcc cgtggccgaa ccaccgccaa tatttggcac tttccctagt catttcatct
    14641 gaaagagtta aaaaatgcga agtcactcca taaaaagtcg ggttgcatca tccgatggag
    14701 tttgacagtt tgttttcaaa taaaataaaa atgtgcaaca tccattgctc aagctaaacc
    14761 agtcactcgg ttagctcagc ttttcttata catttccaaa catattgtca atttccttct
    14821 ggattctgtt ggtatgacaa ctttgttact ctgtcaaaca tgtctacgac tattttaaaa
    14881 ccatttcaga gattattgat gttgtaagtt tttgatatta tttttaactt ctttgcatag
    14941 tgaaagtgag aattatgaaa gacaacttct gctttttaaa aggcacaaaa tctgaaaaaa
    15001 gtttgatttg tggtattaga ggcaaccatt tcagcaaaca tgaacattct cttctggtgt
    15061 ttgggggaaa aagattcatt ctacttcctc aagaaggaag agtgtcacta ttgagctcca
    15121 gggtttggtt agtgcagttg aataataagt tgaattccca aaacttggaa agtcagaagg
    15181 aggaatacac aaaaccgtta aaatgaatgg agaaccaaca tacactcgat gagcactttt
    15241 gtggaagttt caccaactaa gaaaatcttg caaggacacc tcctcttatg aaacacttgg
    15301 taggcacttt tatagcatgc tctttcctac ttcactcctt aaggtgcttc ccacaatatt
    15361 ggaggagttc aagtctgaga aataaaatct gactaagaaa tgatctctgc ttgatttttg
    15421 tactgtgcat gttcttgtat tggttcctag ccttttgtct cctccatttt gtcaagaagt
    15481 tcctcataac tgctatagca agcgacaaca atttctcaca gaccacatgg tgacaagata
    15541 cattaatttc aatccttgtc ttctttttct atttgacttc aacatcaatc ccatagacac
    15601 atggttcttt ctattaattt acaccccttc atactcatca ggctttgtcg tttggagtct
    15661 ctctccatat gaaattgata tgtaatttgg tcccctctat aggtttgtac ttaaaacctc
    15721 caaaacttgt catctttcaa ctttctattt ctttttactc tttgatcttt caacaatcat
    15781 ggaatgggtg tctctttcct tcaaaagcct ttcacaatca ttaattggtg tctcttttca
    15841 atttggcttc tattctcttc ccacttctca ttgttttttt cttttctgtg atgtctctta
    15901 tcttctactg cagcctaacc catcagacag taactactct gataccaagt tgatacaaca
    15961 ctgattttgt atagattttt atgctaaaat tacaaagcgc ctgattttga taatgaccct
    16021 tagaaatcct taaaactctg ttgattttca tctgttgcta acgttcataa aaagagtgga
    16081 actctttctt agttgaaaag atttacctac tcttacagct tctccttgat ttgctatcaa
    16141 tacagagaga tacattgaag agcctatccg aggtatatag atatatttac tgtttataga
    16201 gttttgttga caatgacttt gttactaggg tgaagggtgg gcgcgccgac ccagctttc
//
