LOCUS       sequence-129391-        1166 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_720_S2-1_CGCG-to-ETS21C
FEATURES             Location/Qualifiers
     source          1..1166
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    239..263
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(749..773)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    787..878
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(819..838)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    892..940
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc atggtggctt taccaacagt
       61 accggaatgc cgtttggtat gcgtcttgtg attcaaagtt ggcttattca aaggatatta
      121 acgaaggcag cggcacgtct gctcggctcg aacattcatt cacaactgat gctctcagcc
      181 accccgcgcc cttttatacc gctgcgctcg atccccgggc gagctcgatc gcagatctac
      241 cactttgtac aagaaagctg ggtcgaattc gcccttgccc gggttataaa atacaaaatt
      301 cgaaaatagc acaacgcttg gctttcgtag ctaaacatcg aaaacgaaat ccaaaaacaa
      361 agactcaaag caccctgtag cccggcaata tttccggtta ccccaacaac tacacacatt
      421 tttcaaacga cgaacaaaag ataatgtcga caactaaacc gcagatatgc atatgtttat
      481 gtactccgta caagtcgctg tactacgcac atacacacat agtatattta acaaaacgga
      541 gccaatttcc ggttaattct ccgcatctta tcgcacttag agattccgcc ccacaatcca
      601 aatccatccc gcatcattgg gaatgcagac tgcgtgccag atctggccgt atgtatatcg
      661 tctaaattga tattagcgga cgagagctac ttgacactat ttctcagtgg ctgtcacttc
      721 acagaaattg tgcaaagggc gaattcggag cctgcttttt tgtacaaact tgtggtacct
      781 atcgatagag aaatgttctg gcacctgcac ttgcactggg gacagcctat tttgctagtt
      841 tgttttgttt cgttttgttt tgatggagag cgtatgttag tactatcgat tcacacaaaa
      901 aaccaacaca cagatgtaat gaaaataaag atattttatt gcggccgctc caagtacctc
      961 ccgtacctta atattactta cttatcatgg tagcttgggc tggcgtaata gcgaagaggc
     1021 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc aaattgtaag
     1081 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     1141 ataggccgaa atcggcaaaa tccctt
//
