LOCUS       sequence-129393-        1183 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_722_S2-1_CGCG-to-HEY
FEATURES             Location/Qualifiers
     source          1..1183
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    239..263
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(765..789)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    803..894
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(835..854)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    908..956
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc atggtggctt taccaacagt
       61 accggaatgc cgtttggtat gcgtcttgtg attcaaagtt ggcttattca aaggatatta
      121 acgaaggcag cggcacgtct gctcggctcg aacattcatt cacaactgat gctctcagcc
      181 accccgcgcc cttttatacc gctgcgctcg atccccgggc gagctcgatc gcagatctac
      241 cactttgtac aagaaagctg ggtcgaattc gcccttgccc gggttataaa atacaaaatt
      301 cgaaaatagc acaacgcttg gctttcgtag ctaaacatcg aaaacgaaat ccaaaaacaa
      361 agactcaaag caccctgtag cccggcaatc agccgacacg tgcccctacc ccaacaacta
      421 cacacatttt tcaaacgacg aacaaaagat aatgtcgaca actaaaccgc agatatgcat
      481 atgtttatgt actccgtaca agtcgctgta ctacgcacat acacacatag tatatttaac
      541 aaaacggagc cacagccgac acgtgcccct aattctccgc atcttatcgc acttagagat
      601 tccgccccac aatccaaatc catcccgcat cattgggaat gcagactgcg tgccagatct
      661 ggccgtatgt atatcgtcta aattgatatt agcggacgag agctacttga cactatttct
      721 cagtggctgt cacttcacag aaattgtgca aagggcgaat tcggagcctg cttttttgta
      781 caaacttgtg gtacctatcg atagagaaat gttctggcac ctgcacttgc actggggaca
      841 gcctattttg ctagtttgtt ttgtttcgtt ttgttttgat ggagagcgta tgttagtact
      901 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
      961 ccgctccaag tacctcccgt accttaatat tacttactta tcatggtagc ttgggctggc
     1021 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     1081 aatggcaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     1141 gctcattttt taaccaatag gccgaaatcg gcaaaatccc tta
//
