LOCUS       sequence-129396-        1176 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_757_S2-1_GATA-to-HEY
FEATURES             Location/Qualifiers
     source          1..1176
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..50
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(553..577)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      768..777
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(840..858)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gaacatttct ctatcgatag gtaccacaag tttgtacaaa aaagcaggct ccgaattcgc
       61 cctttgcaca atttctgtga agtgacagcc actgagaaat agtgtcaagt agctctcgtc
      121 cgctaatatc aatttagacg atatacatac ggccagatct ggcacgcagt ctgcattccc
      181 aatgatgcgg gatggatttg gattgtgggg cggaatctct aagtggggca cgtgtcggct
      241 ggcggagaat tacttccgcg acttggctcc gttttgttaa atatactatg tgtgtatgtg
      301 cgtagtacag cgacttgtac ggagtacata aacatatgca tatctgcggt ttagttgtcg
      361 ggggcacgtg tcggctgttg ttcgtcgttt gaaaaatgtg tgtagttgtt ggggtaactg
      421 cgcacaattg ccgggctaca gggtgctttg agtctttgtt tttggatttc gttttcgatg
      481 tttagctacg aaagccaagc gttgtgctat tttcgaattt tgtattttat aacccgggca
      541 agggcgaatt cgacccagct ttcttgtaca aagtggtaga tctgcgatcg agctcgcccg
      601 gggatcgagc gcagcggtat aaaagggcgc ggggtggctg agagcatcag ttgtgaatga
      661 atgttcgagc cgagcagacg tgccgctgcc ttcgttaata tcctttgaat aagccaactt
      721 tgaatcacaa gacgcatacc aaacggcatt ccggtactgt tggtaaagcc accatggaag
      781 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg
      841 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta
      901 cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt
      961 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg
     1021 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg
     1081 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc
     1141 ctaccgtggt gttcgtttcc aaaaaggggt tgcaaa
//
