LOCUS       sequence-129397-        1152 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_757_S2-1_GATA-to-HEY
FEATURES             Location/Qualifiers
     source          1..1152
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    239..263
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(766..790)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    804..895
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(836..855)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    909..957
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc atggtggctt taccaacagt
       61 accggaatgc cgtttggtat gcgtcttgtg attcaaagtt ggcttattca aaggatatta
      121 acgaaggcag cggcacgtct gctcggctcg aacattcatt cacaactgat gctctcagcc
      181 accccgcgcc cttttatacc gctgcgctcg atccccgggc gagctcgatc gcagatctac
      241 cactttgtac aagaaagctg ggtcgaattc gcccttgccc gggttataaa atacaaaatt
      301 cgaaaatagc acaacgcttg gctttcgtag ctaaacatcg aaaacgaaat ccaaaaacaa
      361 agactcaaag caccctgtag cccggcaatt gtgcgcagtt accccaacaa ctacacacat
      421 ttttcaaacg acgaacaaca gccgacacgt gcccccgaca actaaaccgc agatatgcat
      481 atgtttatgt actccgtaca agtcgctgta ctacgcacat acacacatag tatatttaac
      541 aaaacggagc caagtcgcgg aagtaattct ccgccagccg acacgtgccc cacttagaga
      601 ttccgcccca caatccaaat ccatcccgca tcattgggaa tgcagactgc gtgccagatc
      661 tggccgtatg tatatcgtct aaattgatat tagcggacga gagctacttg acactatttc
      721 tcagtggctg tcacttcaca gaaattgtgc aaagggcgaa ttcggagcct gcttttttgt
      781 acaaacttgt ggtacctatc gatagagaaa tgttctggca cctgcacttg cactggggac
      841 agcctatttt gctagtttgt tttgtttcgt tttgttttga tggagagcgt atgttagtac
      901 tatcgattca cacaaaaaac caacacacag atgtaatgaa aataaagata ttttattgcg
      961 gccgctccaa gtacctcccg taccttaata ttacttactt atcatggtag cttgggctgg
     1021 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     1081 gaatggcaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     1141 agctcatttt tt
//
