LOCUS       sequence-129398-        9005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  localization of yeast RNase RMP1 (RNase MRP binding protein) in 
            yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Gill T, Aulds J, Schmitt ME
  TITLE     A specialized processing body that is temporally and asymmetrically 
            regulated during the cell cycle in Saccharomyces cerevisiae.
  JOURNAL   J Cell Biol. 2006 Apr 10;173(1):35-45. Epub 2006 Apr 3.
  PUBMED    16585272
REFERENCE   2  (bases 1 to 9005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2006-04-10"; volume: "173(1)"; pages: "35-45. Epub 
            2006 Apr 3"
COMMENT     Sequence Label: pTG005
FEATURES             Location/Qualifiers
     source          1..9005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             481..1194
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(511..539)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1134..1154
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        2495..2715
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             2716..3519
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    4864..6713
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      6260..6713
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(6747..6769)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6855..6995
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6910..6929
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7010..7027)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7181..7769)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7261..7280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7940..8731,8732..8800))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8563..8582
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8801..8905)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8973..8991
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaattctcaa aacccttaaa aacatatgcc tcaccctaac atattttcca attaaccctc
       61 aatatttctc tgtcacccgg cctctatttt ccattttctt ctttacccgc cacgcgtttt
      121 tttctttcaa atttttttct tccttcttct ttttcttcca cgtcctcttg cataaataaa
      181 taaaccgttt tgaaaccaaa ctcgcctctc tctctccttt ttgaaatatt tttgggtttg
      241 tttgatcctt tccttcccaa tctctcttgt ttaatatata ttcatttata tcacgctctc
      301 tttttatctt cctttttttc ctctctcttg tattcttcct tcccctttct actcaaacca
      361 agaagaaaaa gaaaaggtca atctttgtta aagaatagga tcttctacta catcagcttt
      421 tagatttttc acgcttactg cttttttctt cccaagatcg aaaatttacg gatctcaaag
      481 atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      541 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      601 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      661 gtcactactt tctcttatgg tgttcaatgc ttttcaagat acccagatca tatgaaacag
      721 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      781 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      841 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      901 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      961 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
     1021 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     1081 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     1141 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaaggatcc
     1201 gcaagcttgc tctagataat ctctgctttt gtgcgcgtat gtttatgtat gtacctctct
     1261 ctctatttct atttttaaac caccctctca ataaaataaa aataataaag tatttttaag
     1321 gaaaagacgt gtttaagcac tgactttatc tactttttgt acgttttcat tgatataatg
     1381 tgttttgtct ctcccttttc tacgaaaatt tcaaaaattg accaaaaaaa ggaatatata
     1441 tacgaaaaac tattatattt atatatcata gtgttgataa aaaatgttta tccattggac
     1501 cgtgtatcat atgatcatct cttcatcgct tttcagtttt ataggtttat gcaattgccc
     1561 ttcttgggag gatcttgtag aaccgccatt agaatttgag ttcggcgttt tgataccctg
     1621 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     1681 ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc ccttgagagc cttcaaccca
     1741 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     1801 tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac
     1861 cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac
     1921 gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc
     1981 attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga
     2041 ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg
     2101 ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg
     2161 ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat
     2221 gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt
     2281 gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa
     2341 gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc
     2401 ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct
     2461 ccagcagccg cacgcggcgc atcggggggg gggtttcaat tcaattcatc attttttttt
     2521 tattcttttt tttgatttcg gtttctttga aatttttttg attcggtaat ctccgaacag
     2581 aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt
     2641 gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg
     2701 aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt
     2761 cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca
     2821 ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt
     2881 tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag
     2941 ccgctaaagg cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct
     3001 gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg
     3061 gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag
     3121 gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc
     3181 aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac
     3241 aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac
     3301 gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagacgc attgggtcaa
     3361 cagtatagaa ccgtggatga tgtggtctct acaggatctg acattattat tgttggaaga
     3421 ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc
     3481 tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt ataagtaaat
     3541 gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt attacccggg
     3601 aatctcggtc gtaatgattt ttataatgac gaaaaaaaaa aaattggaaa gaaaaccccc
     3661 cccccgcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac
     3721 ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg
     3781 aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt
     3841 cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt
     3901 ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac
     3961 gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag
     4021 ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt
     4081 gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatt cccccttaca
     4141 cggaggcatc aagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga
     4201 agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac
     4261 atctgtgaat cgcttcacga ccacgctgat gagctttacc gcaggtgggc cattctcatg
     4321 aagaatatct tgaatttatt gtcatattac tagttggtgt ggaagtccat atatcggtga
     4381 tcaatatagt ggttgacatg ctggctagtc aacattgagc cttttgatca tgcaaatata
     4441 ttacggtatt ttacaatcaa atatcaaact taactattga ctttataact tatttaggtg
     4501 gtaacattct tataaaaaag aaaaaaatta ctgcaaaaca gtactagctt ttaacttgta
     4561 tcctaggtta tctatgctgt ctcaccatag agaatattac ctatttcaga atgtatgtcc
     4621 atgattcgcc gggtaaatac atataataca caaatctggc ttaataaagt ctataatata
     4681 tctcataaag aagtgctaaa ttggctagtg ctatatattt ttaagaaaat ttcttttgac
     4741 taagtccata tcgactttgt aaaagttcac tttagcatac atatattaca cgagccagaa
     4801 attgtaactt ttgcctaaaa tcacaaattg caaaatttaa ttgcttgcaa aaggtcacat
     4861 gcttataatc aactttttta aaaatttaaa atactttttt attttttatt tttaaacata
     4921 aatgaaataa tttatttatt gtttatgatt accgaaacat aaaacctgct caagaaaaag
     4981 aaactgtttt gtccttggaa aaaaagcact acctaggagc ggccaaaatg ccgaggcttt
     5041 catagcttaa actctttaca gaaaataggc attatagatc agttcgagtt ttcttattct
     5101 tccttccggt tttatcgtca cagttttaca gtaaataagt atcacctctt agagttaact
     5161 atgagataag caagtatcat ctcatttcat ttacctgaag tcgagtaaac agaaaatcca
     5221 attgttgatg aacctcaatg acttagaact atctatcggc agatcatata aagaggattt
     5281 aggtacctag aggactgtac ctggagtata tatatatata tatatatatt atctcaacta
     5341 tagtccatag aggtttcttt cttgaggcct taaactgcta aagaatgata ttggtggaat
     5401 gcaagcacca atctctcttc tttcgtaact gttcatatac ttcaaaccaa gaatgtaacg
     5461 ggcattgacc catccaaaac cttcagtagc tgccccttta aagtcagcac cttgattacc
     5521 gtattctgct tcaacacgat gaggatctgt tcctcttgtg acatcatatt tttcaaccac
     5581 aataccatta taatcgacaa aagcctttgt catcatgaaa agccatctat aagctagcct
     5641 attcgttaca gttaaataac cataagaacg gaggccttcc caagcaagaa tttgatgggg
     5701 tgcccaacca aatggatagt cccattgtct aattggtctc gaaatagaaa ttgggcctcg
     5761 agaacgctcc gtacatgcag ctaaacctcc aagcatctct aacttgggta gtgctttctc
     5821 caccattttc tgtgcttgct ccttcgtggc aagtccagcc cataatgccc agaatgtagt
     5881 tgcggattcg tatgacgttc tgtgcttgat ttttgtgttg tagtcaaaga aaaaccccga
     5941 ctcgtcatcc cacatatatt tggtaattga tgaggcaacg ctaattatca acatatagat
     6001 tgttatctat ctgcatgaac acgaaatctt tacttgacga cttgaggctg atggtgttta
     6061 tgcaaagaaa ccactgtgtt taatatgtgt cactgtttga tattactgtc agcgtagaag
     6121 ataatagtaa aagcggttaa taagtgtatt tgagataagt gtgataaagt ttttacagcg
     6181 aaaagacgat aaatacaaga aaatgattac gaggatacgg agagaggtat gtacatgtgt
     6241 atttatatac taagctgccg gcggttgttt gcaagaccga gaaaaggcta gcaagaatcg
     6301 ggtcattgta gcgtatgcgc ctgtgaacat tctcttcaac aagtttgatt ccattgcggt
     6361 gaaatggtaa aagtcaaccc cctgcgatgt atattttcct gtacaatcaa tcaaaaagcc
     6421 aaatgattta gcattatctt tacatcttgt tattttacag attttatgtt tagatctttt
     6481 atgcttgctt ttcaaaaggc ctgcaggcaa gtgcacaaac aatacttaaa taaatactac
     6541 tcagtaataa cctatttctt agcatttttg acgaaatttg ctattttgtt agagtctttt
     6601 acaccatttg tctccacacc tccgcttaca tcaacaccaa taacgccatt taatctaagc
     6661 gcatcaccaa cattttctgg cgtcagtcca ccagctaaca taaaatgtaa gctctgcctc
     6721 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     6781 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     6841 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
     6901 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     6961 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
     7021 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7081 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7141 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7201 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7261 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7321 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7381 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7441 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7501 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7561 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7621 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7681 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7741 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7801 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7861 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7921 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7981 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8041 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8101 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8161 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8221 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
     8281 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8341 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8401 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8461 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8521 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     8581 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8641 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8701 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8761 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8821 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8881 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     8941 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     9001 ttcaa
//
