LOCUS       sequence-12941-e       13726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13726)
  AUTHORS   Nodine MD, Bartel DP
  TITLE     MicroRNAs prevent precocious gene expression and enable pattern 
            formation during plant embryogenesis.
  JOURNAL   Genes Dev. 2010 Dec 1. 24(23):2678-92.
  PUBMED    21123653
REFERENCE   2  (bases 1 to 13726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 Dec 1. 24(23):2678-92."
COMMENT     Sequence Label: UB13p::dsSPL10 v01 (SPL10-RNAi)
FEATURES             Location/Qualifiers
     source          1..13726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    66..241
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(281..1306)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(1374..2049)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    3138..3192
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    3217..3241
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3922..3946)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          3953..5301
                     /gene="chsA"
                     /label=chsA intron
                     /note="chalcone synthase A intron from Petunia hybrida"
     protein_bind    5342..5366
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6047..6071)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      6091..6798
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(6823..6840)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6823..6839)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6832..6854)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7042..7066
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8366..8995
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9429..10496
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      10562..10756
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10992..11014)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11100..11240
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11155..11174
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11255..11272)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11426..12014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11506..11525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12101..12895)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13320..13344
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(13430..13682)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
ORIGIN
        1 gtaccactta ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
       61 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
      121 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
      181 aaccctatag gaaccctaat tcccttatct gggaactact cacacattca ttatggagaa
      241 actcgagctt gtcgatcgac agatccggtc ggcatctact ctatttcttt gccctcggac
      301 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
      361 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
      421 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
      481 gatagagttg gtcaagacca atgcggagca tatacgcccg gagtcgtggc gatcctgcaa
      541 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
      601 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
      661 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
      721 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct
      781 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
      841 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
      901 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atagcctccg
      961 cgaccggttg tagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
     1021 gtgaacggcg ggagatgcaa taggtcaggc tctcgctaaa ctccccaatg tcaagcactt
     1081 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
     1141 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
     1201 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
     1261 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc
     1321 cgggatctgc gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt
     1381 gtcctctcca aatgaaatga acttccttat atagaggaag gtcttgcgaa ggatagtggg
     1441 attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg
     1501 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
     1561 cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt
     1621 aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga
     1681 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt
     1741 ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttca
     1801 catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc
     1861 gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt
     1921 cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg
     1981 aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga
     2041 aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg
     2101 agagtgtcgt gctccaccat gttggcaagc tgctctagcc aatacgcaaa ccctcaggga
     2161 gacgcacaga gtggagacgg acgtccaccc caaagcacat acttatcgat ttaaatttca
     2221 tcgaagagat taatatcgaa taatcatata catactttaa atacataaca aattttaaat
     2281 acatatatct ggtatataat taatttttta aagtcatgaa gtatgtatca aatacacata
     2341 tggaaaaaat taactattca taatttaaaa aatagaaaag atacatctag tgaaattagg
     2401 tgcatgtatc aaatacatta ggaaaagggc atatatcttg atctagataa ttaacgattt
     2461 tgatttatgt ataatttcca aatgaaggtt tatatctact tcagagataa caatatactt
     2521 ttatcagaac attcaacaaa gtaacaacca actagagtga aaaatacaca ttgttctcta
     2581 aacatacaaa attgagaaaa gaatctcaaa atttagagaa acaaatctga atttctagaa
     2641 gaaaaaaata attatgcact ttgctattgc tcgaaaaata aatgaaagaa attagacttt
     2701 tttaaaagat gttagactag atatactcaa aagctatcaa aggagtaata ttcttcttac
     2761 attaagtatt ttagttacag tcctgtaatt aaagacacat tttagattgt atctaaactt
     2821 aaatgtatct agaatacata tatttgaatg catcatatac atgtatccga cacaccaatt
     2881 ctcataaaaa gcgtaatatc ctaaactaat ttatccttca agtcaactta agcccaatat
     2941 acattttcat ctctaaaggc ccaagtggca caaaatgtca ggcccaatta cgaagaaaag
     3001 ggcttgtaaa accctaataa agtggcactg gcagagctta cactctcatt ccatcaacaa
     3061 agaaacccta aaagccgcag cgccactgat ttctctcctc caggcgaaga ctcgagtata
     3121 agagctctat ttttacaaca attaccaaca acaacaaaca acaaacaaca ttacaattac
     3181 atttacaatt accatggggc gcgcccaatc gatgattcaa gtttgtacaa aaaagcaggc
     3241 tccgaattcg cccttttgaa aaaggtgaag gaattgaatc tatagttcca gatttcttag
     3301 gctttgagaa agtcagtagt ggctctgcta ctagtttctg gcacactgcc gtatcaaaaa
     3361 gctcgcagtc gacctctatc aactcatcat ctcccgagga caaacgatgc aatcttgcat
     3421 cacaaagttc ccctggagat tcttccagca acatagattt tctccaggtg aaaccatcca
     3481 cagctctcga ggtacctatt gcctcagctg aatcagatct ttgtttgaaa ctaggaaagc
     3541 ggacatactc tgaagaattt tggggtagga acaataatga cctttcagcg gtttctatga
     3601 atttgttgac tccatctgtt gttgctcgga agaaaaccaa atcgtgtggt cagagcatgc
     3661 aagttccgcg ttgccaaatt gatggctgtg agctggatct ctcatcttct aaggattatc
     3721 atcgcaagca tagagtctgc gaaacgcatt caaagtgccc aaaagttgtt gtgagtggcc
     3781 tggaacgtcg tttctgccaa cagtgtagca ggttccatgc tgtctcagaa tttgatgaaa
     3841 agaaacgaag ctgccgcaaa cgtctttctc atcataatgc aaggcgtcgc aagccacaag
     3901 gagtatttaa gggcgaattc gacccagctt tcttgtacaa agtggtaaat gtgtaagaat
     3961 ttcttatgtt acattattac attcaacgtt ttatcttaat tggctcttca tttgattgaa
     4021 atttgacaat tatttcttgt tttttttttt gtcacactct ttttgggttg gggtggccga
     4081 cgaattgtgg gaaggtagaa agaggggagg acttttgtta tactccatta gtaattactg
     4141 tttccgtttc aatttatgtg acaatatttc ctttttagtc ggttccaaaa gaaaatgtca
     4201 gcattataaa caatttaatt ttgaaattac aattttgcca ttaataaaat gatttacaac
     4261 cacaaaagta tctatgagcc tgtttgggtg ggcttataag cagcttattt taagtggctt
     4321 ataagtcaaa aagtgacant ttttgagaag ttagaaaatc ctaacttctc aaaaagtagc
     4381 ttttaagcca cttatgactt ataagtccaa aaatttttaa gttaccaaac atatattaat
     4441 gggtttataa gcttataagc cacttttaag ctcacccaaa cgggttctat gtctcacttt
     4501 agactacaaa ttttaaaagt cttcatttat ttcttaatct ccgtggcgag tnaaactata
     4561 acacataaag tgaaacggag ggaataagat ggagtcataa actaatccaa atctatactc
     4621 tctccgttaa tttgtttttt agtttgattt ggtacattaa taaaacagat ttttcgaagg
     4681 ttataaacac agacagatgt ttcccagcga gctagcaaaa ttccaagatt tctgtcgaaa
     4741 attcgtgtgt ttctagctag tacttgatgt tatctttaac cttttagtaa ttttttgtcc
     4801 ttttctttct atttttcatc ttacaatgaa ttatgagcaa gttccttaag tagcatcaca
     4861 cgtgagatgt tttttatgat attgactaaa tccaatcttt accattcctt aactagtaaa
     4921 atacaacaca tgttaattga tacattgctt aacactgagg ttagaaaatt ttagaaatta
     4981 gttgtccaaa tgctttgaaa ttagaaatct ttaatccctt attttttttt aaaatgtttt
     5041 ttctcactcc aaagaaagag aaactgacat gaaagctcaa aagatcatga atcttactaa
     5101 ctttgtggaa ctaaatgtac atcagaatgt ttctgacatg tgaaaatgaa agctcttaat
     5161 tttcttcttt tatttattga gggtttttgc atgctatgca ttcaatttga gtactttaaa
     5221 gcacctataa acacttactt acacttgcct tggagtttat gttttagtgt tttcttcaca
     5281 tcttttttgg tcaatttgca ggtatttgga tcctaggtga gtctagagag ttaattaaga
     5341 ccccactttg tacaagaaag ctgggtcgaa ttcgccctta aatactcctt gtggcttgcg
     5401 acgccttgca ttatgatgag aaagacgttt gcggcagctt cgtttctttt catcaaattc
     5461 tgagacagca tggaacctgc tacactgttg gcagaaacga cgttccaggc cactcacaac
     5521 aacttttggg cactttgaat gcgtttcgca gactctatgc ttgcgatgat aatccttaga
     5581 agatgagaga tccagctcac agccatcaat ttggcaacgc ggaacttgca tgctctgacc
     5641 acacgatttg gttttcttcc gagcaacaac agatggagtc aacaaattca tagaaaccgc
     5701 tgaaaggtca ttattgttcc taccccaaaa ttcttcagag tatgtccgct ttcctagttt
     5761 caaacaaaga tctgattcag ctgaggcaat aggtacctcg agagctgtgg atggtttcac
     5821 ctggagaaaa tctatgttgc tggaagaatc tccaggggaa ctttgtgatg caagattgca
     5881 tcgtttgtcc tcgggagatg atgagttgat agaggtcgac tgcgagcttt ttgatacggc
     5941 agtgtgccag aaactagtag cagagccact actgactttc tcaaagccta agaaatctgg
     6001 aactatagat tcaattcctt cacctttttc aaaagggcga attcggagcc tgcttttttg
     6061 tacaaacttg cgggactagt ccctagagtc ctgctttaat gagatatgcg agacgcctat
     6121 gatcgcatga tatttgcttt caattctgtt gtgcacgttg taaaaaacct gagcatgtgt
     6181 agctcagatc cttaccgccg gtttcggttc attctaatga atatatcacc cgttactatc
     6241 gtatttttat gaataatatt ctccgttcaa tttactgatt gtaccctact acttatatgt
     6301 acaatattaa aatgaaaaca atatattgtg ctgaataggt ttatagcgac atctatgata
     6361 gagcgccaca ataacaaaca attgcgtttt attattacaa atccaatttt aaaaaaagcg
     6421 gcagaaccgg tcaaacctaa aagactgatt acataaatct tattcaaatt tcaaaagtgc
     6481 cccaggggct agtatctacg acacaccgag cggcgaacta ataacgctca ctgaagggaa
     6541 ctccggttcc ccgccggcgc gcatgggtga gattccttga agttgagtat tggccgtccg
     6601 ctctaccgaa agttacgggc accattcaac ccggtccagc acggcggccg ggtaaccgac
     6661 ttgctgcccc gagaattatg cagcattttt ttggtgtatg tgggccccaa atgaagtgca
     6721 ggtcaaacct tgacagtgac gacaaatcgt tgggcgggtc cagggcgaat tttgcgacaa
     6781 catgtcgagg ctcagcagga cctgcaggca tgcaagcttg gcactggccg tcgttttaca
     6841 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     6901 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     6961 cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg tttcccgcct
     7021 tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag
     7081 cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt
     7141 gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc
     7201 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg
     7261 tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt
     7321 gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc
     7381 atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag
     7441 gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag
     7501 aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc
     7561 cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta
     7621 ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg
     7681 tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc
     7741 gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc
     7801 cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc
     7861 gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat
     7921 cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg
     7981 cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag
     8041 aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt
     8101 tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg
     8161 cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg
     8221 cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat
     8281 gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa
     8341 ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg
     8401 gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga
     8461 tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga
     8521 agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc
     8581 catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt
     8641 gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat
     8701 atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag
     8761 gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt
     8821 tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt
     8881 gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga
     8941 cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa
     9001 tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca
     9061 cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc
     9121 aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc
     9181 aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa
     9241 tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca
     9301 accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt
     9361 aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc
     9421 ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
     9481 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
     9541 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
     9601 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
     9661 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     9721 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     9781 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
     9841 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     9901 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     9961 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
    10021 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
    10081 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
    10141 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
    10201 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
    10261 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
    10321 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
    10381 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
    10441 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
    10501 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
    10561 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
    10621 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
    10681 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
    10741 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
    10801 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
    10861 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
    10921 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
    10981 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
    11041 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
    11101 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
    11161 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
    11221 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    11281 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    11341 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    11401 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    11461 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    11521 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    11581 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    11641 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    11701 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    11761 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    11821 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    11881 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    11941 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    12001 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    12061 aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta
    12121 aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct
    12181 cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat
    12241 accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt
    12301 tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct
    12361 tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc
    12421 agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc
    12481 ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga
    12541 tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg
    12601 agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa
    12661 agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc
    12721 gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt
    12781 cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc
    12841 agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt
    12901 atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac
    12961 gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc
    13021 aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg
    13081 gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat
    13141 catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg
    13201 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
    13261 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
    13321 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg
    13381 acgtttttaa tgtactgaat taacgccgaa ttaattcgag ctcggatctg ataatttatt
    13441 tgaaaattca taagaaaagc aaacgttaca tgaattgatg aaacaataca aagacagata
    13501 aagccacgca catttaggat attggccgag attactgaat attgagtaag atcacggaat
    13561 ttctgacagg agcatgtctt caattcagcc caaatggcag ttgaaatact caaaccgccc
    13621 catatgcagg agcggatcat tcattgtttg tttggttgcc tttgccaaca tgggagtcca
    13681 agattctgca gttagatctc ggtgacgggc aggaccggac ggggcg
//
