LOCUS       sequence-129415-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-His tagged nonglycosylated human serum transferrin 
            unable to release iron from the N-lobe or the C-lobe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Halbrooks PJ, He QY, Briggs SK, Everse SJ, Smith VC, MacGillivray 
            RT, Mason AB
  TITLE     Investigation of the mechanism of iron release from the C-lobe of 
            human serum transferrin: mutational analysis of the role of a pH 
            sensitive triad.
  JOURNAL   Biochemistry. 2003 Apr 8;42(13):3701-7.
  PUBMED    12667060
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2003-04-8"; volume: "42(13)"; pages: 
            "3701-7"
COMMENT     Sequence Label: pNUT N6His K206E/K534A hTFNG
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             106..123
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             124..135
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gcgtcacccc tagagtcgac gcggccgccc gggaagatga ggctcgccgt gggagccctg
       61 ctggtctgcg ccgtcctagg gctgtgtctg gctgtccctg ataaacatca tcatcatcat
      121 catatcgagg gaagggtccc tgataaaact gtgagatggt gtgcagtgtc ggagcatgag
      181 gccactaagt gccagagttt ccgcgaccat atgaaaagcg tcattccatc cgatggtccc
      241 agtgttgctt gtgtgaagaa agcctcctac cttgattgca tcagggccat tgcggcaaac
      301 gaagcggatg ctgtgacact ggatgcaggt ttggtgtatg atgcttacct ggctcccaat
      361 aacctgaagc ctgtggtggc agagttctat gggtcaaaag aggatccaca gactttctat
      421 tatgctgttg ctgtggtgaa gaaggatagt ggcttccaga tgaaccagct tcgaggcaag
      481 aagtcctgcc acacgggtct aggcaggtcc gctgggtgga acatccccat aggcttactt
      541 tactgtgact tacctgagcc acgtaaacct cttgagaaag cagtggccaa tttcttctcg
      601 ggcagctgtg ccccttgtgc ggatgggacg gacttccccc agctgtgtca actgtgtcca
      661 gggtgtggct gctccaccct taaccaatac ttcggctact cgggagcctt caagtgtctg
      721 aaggatggtg ctggggatgt ggcctttgtc gagcactcga ctatatttga gaacttggca
      781 aacaaggctg acagggacca gtatgagctg ctttgcctgg acaacacccg gaagccggta
      841 gatgaataca aggactgcca cttggcccag gtcc
//
