LOCUS       sequence-129420-        1195 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_975_Nhe2_EcR-mut
FEATURES             Location/Qualifiers
     source          1..1195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..50
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(505..529)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      720..729
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(792..810)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gaacatttct ctatcgatag gtaccacaag tttgtacaaa aaagcaggct ccgaattcgc
       61 ccttccagtt ggcctgcgat aagatctgat ttgcattatt ggcgaaattt tattagcagc
      121 agtagcgaaa gcttatctgt cgagtgcgga atgactaaag actcccaatt gagcaattgg
      181 tgtgtacatc gagtgtaatt ttcgagatgc tgataaatct ctgagggatt tgtacgagtc
      241 ggattcagta attgtcccga cttgagcaat tgcatccgtt ggtattcgct atctgctgga
      301 atgagtgtcg cccatctcat tatataaaat tggtagtcaa tttaggatga tgcctcatgc
      361 atttttccgt ctccatattc aattgttcta accagtggcc taggccttcc acaaattcaa
      421 tttggtgttt taatttaagt atcttgactg tgattacaga gtgttgacag agtacagcaa
      481 tatttgaaaa caagggcgaa ttcgacccag ctttcttgta caaagtggta gatctgcgat
      541 cgagctcgcc cggggatcga gcgcagcggt ataaaagggc gcggggtggc tgagagcatc
      601 agttgtgaat gaatgttcga gccgagcaga cgtgccgctg ccttcgttaa tatcctttga
      661 ataagccaac tttgaatcac aagacgcata ccaaacggca ttccggtact gttggtaaag
      721 ccaccatgga agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag
      781 atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa
      841 caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa
      901 tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg
      961 tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg
     1021 gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg
     1081 gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg
     1141 tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa acgat
//
