LOCUS       sequence-129421-        1085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_975_Nhe2_EcR-mut
FEATURES             Location/Qualifiers
     source          1..1085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    238..262
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(717..741)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    755..846
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(787..806)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    860..908
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 aatggcgccg ggcctttctt tatgtttttg gcgtcttcca tggtggcttt accaacagta
       61 ccggaatgcc gtttggtatg cgtcttgtga ttcaaagttg gcttattcaa aggatattaa
      121 cgaaggcagc ggcacgtctg ctcggctcga acattcattc acaactgatg ctctcagcca
      181 ccccgcgccc ttttataccg ctgcgctcga tccccgggcg agctcgatcg cagatctacc
      241 actttgtaca agaaagctgg gtcgaattcg cccttgtttt caaatattgc tgtactctgt
      301 caacactctg taatcacagt caagatactt aaattaaaac accaaattga atttgtggaa
      361 ggcctaggcc actggttaga acaattgaat atggagacgg aaaaatgcat gaggcatcat
      421 cctaaattga ctaccaattt tatataatga gatgggcgac actcattcca gcagatagcg
      481 aataccaacg gatgcaattg ctcaagtcgg gacaattact gaatccgact cgtacaaatc
      541 cctcagagat ttatcagcat ctcgaaaatt acactcgatg tacacaccaa ttgctcaatt
      601 gggagtcttt agtcattccg cactcgacag ataagctttc gctactgctg ctaataaaat
      661 ttcgccaata atgcaaatca gatcttatcg caggccaact ggaagggcga attcggagcc
      721 tgcttttttg tacaaacttg tggtacctat cgatagagaa atgttctggc acctgcactt
      781 gcactgggga cagcctattt tgctagtttg ttttgtttcg ttttgttttg atggagagcg
      841 tatgttagta ctatcgattc acacaaaaaa ccaacacaca gatgtaatga aaataaagat
      901 attttattgc ggccgctcca agtacctccc gtaccttaat attacttact tatcatggta
      961 gcttgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     1021 gcctgaatgg cgaatggcaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     1081 tttgt
//
