LOCUS       sequence-12944-e       12198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12198)
  AUTHORS   Nodine MD, Bartel DP
  TITLE     MicroRNAs prevent precocious gene expression and enable pattern 
            formation during plant embryogenesis.
  JOURNAL   Genes Dev. 2010 Dec 1. 24(23):2678-92.
  PUBMED    21123653
REFERENCE   2  (bases 1 to 12198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 Dec 1. 24(23):2678-92."
COMMENT     Sequence Label: pMRS156.2 (miR156 sensor)
FEATURES             Location/Qualifiers
     source          1..12198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(576..578,579..581,582..1292)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(621..642)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(882..901)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1229..1250
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1613..1630)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1613..1629)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1622..1644)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2017..2035)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      2222..2474
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2496..2520
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3820..4449
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4883..5950
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6016..6210
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6554..6694
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6709..6726)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6880..7468)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6960..6979)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7555..8349)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8774..8798
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8876..9050
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9090..10115)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10183..10860)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    11051..11072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11087..11092,11093..11110,11111..11117)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11125..11141
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11130..11152
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11149..11165
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11149..11165
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    11185..11209
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(12188..12198,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggttcgata attctcaaca caacatatac aaaacaaacg aatctcaagc
       61 aatcaagcat tctacttcta ttgcagcaat ttaaatcatt tcttttaaag caaaagcatg
      121 ccatggaatc caccggaaaa gtgaagaaag ctttcggagg aagaaaacca cctggtgccc
      181 caaaaaccaa atcggtttcg aaatcgatga aagccggtct tcaattccca gtgggaagaa
      241 tcactcgttt cctgaagaaa ggacgatacg ctcagagact tggtggtggt gctccggttt
      301 acatggccgc cgttcttgaa tacctcgccg cagaagttct ggagcttgct ggtaacgctg
      361 cgagagataa caagaaatca aggataattc cgaggcatct tcttctcgcg ataaggaacg
      421 atgaagaatt ggggaaactt ctgagtggtg tcacaatcgc tcacggtggt gttttgccta
      481 acatcaactc tgttctattg cctaagaagt ctgccactaa accagctgaa gaaaaggcta
      541 ccaaatcacc agtcaagtct ccaaagaaag ctgccatggt gagcaagggc gaggagctgt
      601 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      661 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      721 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc acctacggcg
      781 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      841 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      901 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      961 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     1021 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     1081 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     1141 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     1201 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1261 ggatcactca cggcatggac gagctgtaca aggggatcct ctagacgaat gtagtacgta
     1321 ttctccgaac tgaagcacct gagacgtgta atgtcgggcc atctcatacg tacggctaga
     1381 gtccgcaaaa atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat
     1441 aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt
     1501 tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa
     1561 tttctaattc ctaaaaccaa aatccagtga cctgcaggca tgcaagcttg gcactggccg
     1621 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     1681 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     1741 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     1801 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     1861 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     1921 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     1981 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     2041 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     2101 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     2161 gacaataacc ctgataaatg cttcaataat gtgtgaattg gtgaccagct cgaatttccc
     2221 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     2281 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     2341 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     2401 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     2461 tatgttacta gatcgggaat taaactatca gtgtttgaca ggatatattg gcgggtaaac
     2521 ctaagagaaa agagcgttta ttagaataac ggatatttaa aagggcgtga aaaggtttat
     2581 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
     2641 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
     2701 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
     2761 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
     2821 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
     2881 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
     2941 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     3001 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
     3061 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta
     3121 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
     3181 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg
     3241 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga
     3301 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac
     3361 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc
     3421 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc
     3481 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca
     3541 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt
     3601 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc
     3661 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg
     3721 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
     3781 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
     3841 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa
     3901 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
     3961 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
     4021 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
     4081 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
     4141 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
     4201 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc
     4261 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
     4321 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
     4381 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
     4441 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
     4501 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
     4561 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
     4621 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag
     4681 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
     4741 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
     4801 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc
     4861 aagcccgagg aatcggcgtg acggtcgcaa accatccggc ccggtacaaa tcggcgcggc
     4921 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
     4981 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga
     5041 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
     5101 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
     5161 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
     5221 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
     5281 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
     5341 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
     5401 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
     5461 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
     5521 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
     5581 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga
     5641 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
     5701 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
     5761 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
     5821 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
     5881 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
     5941 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
     6001 aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
     6061 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
     6121 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
     6181 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct
     6241 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
     6301 tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
     6361 cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg
     6421 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     6481 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     6541 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     6601 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     6661 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     6721 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6781 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6841 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6901 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6961 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7021 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7081 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7141 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7201 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7261 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7321 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7381 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7441 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7501 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta ggtactaaaa
     7561 caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa
     7621 gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca
     7681 gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact
     7741 tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag
     7801 ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg
     7861 agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc
     7921 caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga
     7981 gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc
     8041 ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc
     8101 atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat
     8161 catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt
     8221 taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc
     8281 cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat
     8341 tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct
     8401 aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag
     8461 aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg
     8521 attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct
     8581 accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata
     8641 gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc
     8701 ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
     8761 gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta
     8821 ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt cgggggatct
     8881 ggattttagt actggatttt ggttttagga attagaaatt ttattgatag aagtatttta
     8941 caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga aaccctatag
     9001 gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa ctcgagcttg
     9061 tcgatcgaca gatccggtcg gcatctactc tatttctttg ccctcggacg agtgctgggg
     9121 cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt
     9181 ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat
     9241 cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg
     9301 tcaagaccaa tgcggagcat atacgcccgg agtcgtggcg atcctgcaag ctccggatgc
     9361 ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag
     9421 atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct
     9481 gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc
     9541 cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac
     9601 gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg
     9661 catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac
     9721 ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tagcctccgc gaccggttgt
     9781 agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg
     9841 gagatgcaat aggtcaggct ctcgctaaac tccccaatgt caagcacttc cggaatcggg
     9901 agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag
     9961 ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct
    10021 tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc
    10081 tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc attgcccccc gggatctgcg
    10141 aaagctcgag agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa
    10201 atgaaatgaa cttccttata tagaggaagg tcttgcgaag gatagtggga ttgtgcgtca
    10261 tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg
    10321 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
    10381 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
    10441 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
    10501 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
    10561 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttatc acatcaatcc
    10621 acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg
    10681 ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg
    10741 caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag
    10801 atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca
    10861 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
    10921 tgctccacca tgttggcaag ctgctctagc caatacgcaa accgcctctc cccgcgcgtt
    10981 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    11041 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    11101 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    11161 atgaccatga ttacgaatta tcaaacaagt ttgtacaaaa aagcaggctc cgcggccgcc
    11221 cccttcaccc caaagcacat acttatcgat ttaaatttca tcgaagagat taatatcgaa
    11281 taatcatata catactttaa atacataaca aattttaaat acatatatct ggtatataat
    11341 taatttttta aagtcatgaa gtatgtatca aatacacata tggaaaaaat taactattca
    11401 taatttaaaa aatagaaaag atacatctag tgaaattagg tgcatgtatc aaatacatta
    11461 ggaaaagggc atatatcttg atctagataa ttaacgattt tgatttatgt ataatttcca
    11521 aatgaaggtt tatatctact tcagagataa caatatactt ttatcagaac attcaacaaa
    11581 gtaacaacca actagagtga aaaatacaca ttgttctcta aacatacaaa attgagaaaa
    11641 gaatctcaaa atttagagaa acaaatctga atttctagaa gaaaaaaata attatgcact
    11701 ttgctattgc tcgaaaaata aatgaaagaa attagacttt tttaaaagat gttagactag
    11761 atatactcaa aagctatcaa aggagtaata ttcttcttac attaagtatt ttagttacag
    11821 tcctgtaatt aaagacacat tttagattgt atctaaactt aaatgtatct agaatacata
    11881 tatttgaatg catcatatac atgtatccga cacaccaatt ctcataaaaa gcgtaatatc
    11941 ctaaactaat ttatccttca agtcaactta agcccaatat acattttcat ctctaaaggc
    12001 ccaagtggca caaaatgtca ggcccaatta cgaagaaaag ggcttgtaaa accctaataa
    12061 agtggcactg gcagagctta cactctcatt ccatcaacaa agaaacccta aaagccgcag
    12121 cgccactgat ttctctcctc caggcgaaga gtgctctctc tcttctgtca aaagggtggg
    12181 cgcgccgacc cagctttc
//
