LOCUS       sequence-129440-        1167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_889_Nhe2_EcR-to-Ets96B_RB
FEATURES             Location/Qualifiers
     source          1..1167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..50
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(469..493)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      684..693
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(756..774)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gaacatttct ctatcgatag gtaccacaag tttgtacaaa aaagcaggct tcctgcatca
       61 ccggaagtac caggtccatt attcataaac cggaagtaca acagagacta ctcgcgaatc
      121 cgaagaccca aaccgaatat tactatcact cattcgtaaa atcaaagttc accccaagaa
      181 tcgactcgat tcaagtcact aacaaggttc ttacaggcct tcaaaaatta gactcatgga
      241 ttatgggtag agccgttgaa cacttgaatg agtgtcttaa tagggtggcc acgttcgtac
      301 cggaagtact attaggggac ggtaccctgg aagaagcttt cagtgcaaag gacgagttaa
      361 tagtttcaat acccggtcaa taataagcac cgacacgaaa caattataat gattatacgt
      421 tcttggtctc tgttaacctt aatccagata ataggtcgca aattaaagac ccagctttct
      481 tgtacaaagt ggtagatctg cgatcgagct cgcccgggga tcgagcgcag cggtataaaa
      541 gggcgcgggg tggctgagag catcagttgt gaatgaatgt tcgagccgag cagacgtgcc
      601 gctgccttcg ttaatatcct ttgaataagc caactttgaa tcacaagacg cataccaaac
      661 ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata aagaaaggcc
      721 cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga
      781 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca
      841 tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg
      901 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc
      961 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg
     1021 aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa
     1081 aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta
     1141 tcatggattc taaaacggat taccagg
//
