LOCUS       sequence-129441-        1122 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_889_Nhe2_EcR-to-Ets96B_RB
FEATURES             Location/Qualifiers
     source          1..1122
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    239..263
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(682..706)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    720..811
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(752..771)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    825..873
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc atggtggctt taccaacagt
       61 accggaatgc cgtttggtat gcgtcttgtg attcaaagtt ggcttattca aaggatatta
      121 acgaaggcag cggcacgtct gctcggctcg aacattcatt cacaactgat gctctcagcc
      181 accccgcgcc cttttatacc gctgcgctcg atccccgggc gagctcgatc gcagatctac
      241 cactttgtac aagaaagctg ggtctttaat ttgcgaccta ttatctggat taaggttaac
      301 agagaccaag aacgtataat cattataatt gtttcgtgtc ggtgcttatt attgaccggg
      361 tattgaaact attaactcgt cctttgcact gaaagcttct tccagggtac cgtcccctaa
      421 tagtacttcc ggtacgaacg tggccaccct attaagacac tcattcaagt gttcaacggc
      481 tctacccata atccatgagt ctaatttttg aaggcctgta agaaccttgt tagtgacttg
      541 aatcgagtcg attcttgggg tgaactttga ttttacgaat gagtgatagt aatattcggt
      601 ttgggtcttc ggattcgcga gtagtctctg ttgtacttcc ggtttatgaa taatggacct
      661 ggtacttccg gtgatgcagg aagcctgctt ttttgtacaa acttgtggta cctatcgata
      721 gagaaatgtt ctggcacctg cacttgcact ggggacagcc tattttgcta gtttgttttg
      781 tttcgttttg ttttgatgga gagcgtatgt tagtactatc gattcacaca aaaaaccaac
      841 acacagatgt aatgaaaata aagatatttt attgcggccg ctccaagtac ctcccgtacc
      901 ttaatattac ttacttatca tggtagcttg ggctggcgta atagcgaaga ggcccgcacc
      961 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcaaattgt aagcgttaat
     1021 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     1081 gaaatcggca aaatccctta taaatcaaaa gaatagaccg ag
//
