LOCUS       sequence-129445-        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_240_pAGW_V5
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    107..231
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(272..577)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(374..393)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aaattttata tttaccttag agctttaaat ctctgtaggt agtttgtcca attatgtcac
       61 accacagaag taaggttcct tcacaaagat cctgctagct tacgtcacca ctttgtacaa
      121 gaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
      181 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggtcgacctg
      241 cagactggct gtgtataagg gagcctgaca tttatattcc ccagaacatc aggttaatgg
      301 cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg ataacggaga
      361 ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg atatgcacca
      421 ccgggtaaag ttcacggggg actttatctg acagcagacg tgcactggcc agggggatca
      481 ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac agacgataac
      541 ggctctctct tttataggtg taaaccttaa actgcatttc accagcccct gttctcgtca
      601 gcaaaagagc cgttcatttc aataaaccgg gcgacctcag ccatcccttc ctgattttcc
      661 gctttccagc gttcggcacg cagacgacgg gcttcattct gcatggttgt gcttaccaga
      721 ccggagatat tgacatcata tatgccttga gcaactgata gctgtcgctg tcaactgtca
      781 ctgtaatacg ctgcttcata gcatacctct ttttgacata cttcgggtat acatatcagt
      841 atatattctt ataccgcaaa aatcagcgcg caaatacgca tactgttatc tggcttttag
      901 taagccggat cctctagatt acgccccgcc tgccactcat cgcagtactg ttgtaattca
      961 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
     1021 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag
     1081 aagttgtcca t
//
