LOCUS       sequence-129450-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_91_4xUAS_dCP
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    243..267
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(372..396)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    443..534
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(475..494)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    548..596
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggcttta ccaacagtac
       61 cggaatgccg tttggtatgc gtcttgtgat tcaaagttgg cttattcaaa ggatattaac
      121 gaaggcagcg gcacgtctgc tcggctcgaa cattcattca caactgatgc tctcagccac
      181 cccgcgccct tttataccgc tgcgctcgat ccccgggcga gctcgatcgc agatcttgat
      241 ataccacttt gtacaagaaa gctgggtcct cggaggacag tactccgctc ggaggacagt
      301 actccgacct gcaggcatgc aagcttcccg ctcggaggac agtactccgc tcggaggaca
      361 gtactcccgg aagcctgctt ttttgtacaa acttgtttga tatcctcgag gctagcgagc
      421 tcaggtaccg gccagttagg ccagagaaat gttctggcac ctgcacttgc actggggaca
      481 gcctattttg ctagtttgtt ttgtttcgtt ttgttttgat ggagagcgta tgttagtact
      541 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
      601 cctgtccaat acctcccgta ccttaatatt acttacttat ccttgagaga cgtactagta
      661 accctgataa atgcttcaat aatattgaaa aaggacgagt atgagcatcc aacattttcg
      721 tgtcgcactc attcccttct ttgcggcatt ttgcttgcct gtttttgcac accccgaaac
      781 gctggtgaaa gtaaaagatg ctgaagatca actgggtgca agagtgggct atatcgaact
      841 ggatctcaat agcggcaaga tccttgagtc tttccgcccc gaagaacgat tcccgatgat
      901 gagcactttc aaagtactgc tatgtggcgc ggtgttgtcc cgtatagacg ccgggcaaga
      961 gcagcttggt cgccgtatac actactcaca aaacgacttg gttgagtact cgccggtcac
     1021 ggaaaagcat cttacggatg gcatgacggt aagagaattg tgtagtgctg ccattaccat
     1081 gagcgacaac ac
//
