LOCUS       sequence-129454-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_MC19_Trl-2xUAS_dCP
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(159..183)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      380..389
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(452..470)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 aacatttctc tggcctaact ggccggtacc tgagctcgct agcctcgagg atatcaaaca
       61 agtttgtaca aaaaagcagg cttcttgctc tctctgtttg ctctctcgcc gccgcccgga
      121 gtactgtcct ccgagtgtcg gagtactgtc ctccgaggac ccagctttct tgtacaaagt
      181 ggtatatcaa gatctgcgat cgagctcgcc cggggatcga gcgcagcggt ataaaagggc
      241 gcggggtggc tgagagcatc agttgtgaat gaatgttcga gccgagcaga cgtgccgctg
      301 ccttcgttaa tatcctttga ataagccaac tttgaatcac aagacgcata ccaaacggca
      361 ttccggtact gttggtaaag ccaccatgga agacgccaaa aacataaaga aaggcccggc
      421 gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag
      481 atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac
      541 ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct
      601 gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt
      661 gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg
      721 tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg
      781 gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat
      841 ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct
      901 acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat
      961 tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca
     1021 tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat
     1081 tccggatact gc
//
