LOCUS       sequence-129455-        1123 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_MC19_Trl-2xUAS_dCP
FEATURES             Location/Qualifiers
     source          1..1123
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    243..267
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(344..368)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    415..506
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(447..466)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    520..568
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggcttta ccaacagtac
       61 cggaatgccg tttggtatgc gtcttgtgat tcaaagttgg cttattcaaa ggatattaac
      121 gaaggcagcg gcacgtctgc tcggctcgaa cattcattca caactgatgc tctcagccac
      181 cccgcgccct tttataccgc tgcgctcgat ccccgggcga gctcgatcgc agatcttgat
      241 ataccacttt gtacaagaaa gctgggtcct cggaggacag tactccgaca ctcggaggac
      301 agtactccgg gcggcggcga gagagcaaac agagagagca agaagcctgc ttttttgtac
      361 aaacttgttt gatatcctcg aggctagcga gctcaggtac cggccagtta ggccagagaa
      421 atgttctggc acctgcactt gcactgggga cagcctattt tgctagtttg ttttgtttcg
      481 ttttgttttg atggagagcg tatgttagta ctatcgattc acacaaaaaa ccaacacaca
      541 gatgtaatga aaataaagat attttattgc ggcctgtcca atacctcccg taccttaata
      601 ttacttactt atccttgaga gacgtactag taaccctgat aaatgcttca ataatattga
      661 aaaaggacga gtatgagcat ccaacatttt cgtgtcgcac tcattccctt ctttgcggca
      721 ttttgcttgc ctgtttttgc acaccccgaa acgctggtga aagtaaaaga tgctgaagat
      781 caactgggtg caagagtggg ctatatcgaa ctggatctca atagcggcaa gatccttgag
      841 tctttccgcc ccgaagaacg attcccgatg atgagcactt tcaaagtact gctatgtggc
      901 gcggtgttgt cccgtataga cgccgggcaa gagcagcttg gtcgccgtat acactactca
      961 caaaacgact tggttgagta ctcgccggtc acggaaaagc atcttacgga tggcatgacg
     1021 gtaagagaat tgtgtagtgc tgccattacc atgagcgaca acaccgcggc caacttactt
     1081 ctgacaacga tcggaggccc taaggagctg actgcatttc ttc
//
