LOCUS       sequence-129458-       12721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd gen lentiviral vector. tet/dox controllable expression of 
            FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12721)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 12721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G
FEATURES             Location/Qualifiers
     source          1..12721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2413..2530
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2572..2886
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2580..2598
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2616..2634
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2651..2669
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2687..2705
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2723..2741
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2758..2776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2794..2812
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2859..2883
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2897..2906
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2906..2929
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2930..2956
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4105..5314
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5151..5170
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5327..5944,5945..5950,5951..5986,5987..5989,5990..6025,
                     6026..6028,6029..6064,6065..6073)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    join(6085..6659,6660..6662,6663..6671)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6252..6269)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6479..6498
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6672..6674,6675..6677,6678..7391)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6672..6674,6675..6677,6678..7388)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6717..6738)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6978..6997)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7325..7346
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7415..8003
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7468..7488)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7886..7897)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8522..8702
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8728..8745)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8734..8958
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9004..9432
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9091..9110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9301..9322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9441..9461)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9446..9776
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9627..9762
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9689..9708
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9824..9871
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9890..10264
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10394..10515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10431..10450)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10485..10504
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10564..10580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10564..10580)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10577..10599)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10588..10604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10612..10618,10619..10636,10637..10642))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10657..10678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10795..10812)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10966..11554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11046..11065)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11725..12516,12517..12585))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12348..12367
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12586..12690)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctac aaatggcagt
     2401 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2461 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2521 tcaaaatttt cgggtttatt acagggacag cagaaattca ctttgaattg cgagtttact
     2581 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg
     2641 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     2701 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc
     2761 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
     2821 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
     2881 atcgcctgga gaattcgcca ccatggacta caaggacgac gatgacaagt acccttatga
     2941 cgtgcccgat tacgcttctg gtggcggtgg ctcgggcgga ggtgggtcgg gtggcggcgg
     3001 atcaggttcc atgcagtcca aacgggagtg cgagcaatgg tgtgagaggg tgaatccaga
     3061 gaacaaggca gccctggagg cgtgggtaag ggagactggc atccgcctgg tgcaggtcaa
     3121 cgggcaaagg aagtatggag ggccaccccc aggctgggtg ggcagcccgc caccgtcagg
     3181 gtccgaggtg tatatcggac gacttccgca ggacgtgtac gagcaccagc taatcccact
     3241 cttccagcgc gtggggcgcc tctatgagtt ccgcctaatg atgaccttca gtggtttgaa
     3301 ccgcggcttt gccgcggcgc gctacagctc gcggcgcggt gcccaggccg ccattgctac
     3361 gctgcacaac caccagctgc ggccttcttg ccagctcctg gtgtgccgaa gcaccgagaa
     3421 gtgcgagctg acggtggatg gtctcccctt atcactcaac cgccgagcgt tactgctcgc
     3481 cctgcaaccg ttcggtccct gcctccagga gacactgctg ctacccagcc ctgggtctgc
     3541 gccctctcag attgcgctgc tcaagttcag tacgcaccga gctgctgcta tggccaaaaa
     3601 ggccctggta gaaggtcagt cacgcctctg tggagagcag gtggctgtgg agtggctcaa
     3661 gccagaccta aagcagcact tccgccagca gcttgcaggt ccctcactgc ggttcctcag
     3721 gccagatgtc agccaactaa cccagaccag ggagaagctg gggtcccaag gggctcgggc
     3781 tgccctgcaa ctgctgtgtc aacggatgaa gctaggcagc ccagtgttcc tgaccaagtg
     3841 tttaggaaca gggcctgctg gctggcatcg cttttggtac caggtggtga tccctgggca
     3901 tccagtgcct ttcagtggcc tcatttgggt tgtgttggcc tctgattggc aggatgggca
     3961 tgaggtggcc aaggatgctg tgtccgcaca gctgctggag gcactgagtg aacctaggac
     4021 cagcttgtgg tctcctgggg ctgaggctgg taccatggtg aaacagtgag gatcctctag
     4081 tcagctgacg cgtgctagcg atctggcctc cgcgccgggt tttggcgcct cccgcgggcg
     4141 cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcaggagc gtcctgatcc
     4201 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     4261 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     4321 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     4381 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     4441 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     4501 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     4561 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     4621 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     4681 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     4741 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     4801 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     4861 tcgtctgttg cgggggcggc agttatgcgg tgccgttggg cagtgcaccc gtacctttgg
     4921 gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg
     4981 gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg
     5041 cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt
     5101 gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg
     5161 gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt
     5221 tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc
     5281 taaattctgg ccgtttttgg cttttttgtt agacaagctt gtcaccatgt ctagactgga
     5341 caagagcaaa gtcataaacg gcgctctgga attactcaat ggagtcggta tcgaaggcct
     5401 gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt
     5461 gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac
     5521 ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc
     5581 attccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc
     5641 aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt
     5701 ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt
     5761 attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc
     5821 tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc
     5881 cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag
     5941 cggcgggccg gccgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct
     6001 tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat
     6061 gctccccggg taactcgaga tccgcccctc tccctccccc ccccctaacg ttactggccg
     6121 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     6181 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     6241 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     6301 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     6361 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     6421 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     6481 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     6541 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     6601 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     6661 tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     6721 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     6781 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     6841 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     6901 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     6961 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     7021 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     7081 tccttgggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     7141 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     7201 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     7261 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     7321 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     7381 tgtacaagta actcgaggga tcaagcttat cgataatcaa cctctggatt acaaaatttg
     7441 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7501 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7561 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7621 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7681 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7741 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7801 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     7861 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7921 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     7981 ctccctttgg gccgcctccc cgcatcgata ccgtcgagac ctggaaaaac atggagcaat
     8041 cacaagtagc aacacagcag ctaccaatgc tgcttgtgcc tggctagaag cacaagagga
     8101 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     8161 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     8221 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     8281 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
     8341 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
     8401 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
     8461 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
     8521 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     8581 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     8641 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8701 cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     8761 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     8821 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     8881 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     8941 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     9001 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     9061 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     9121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat
     9181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     9241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     9301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     9361 tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     9421 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     9481 cccaggcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     9541 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     9601 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     9661 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     9721 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     9781 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     9841 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
     9901 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     9961 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
    10021 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
    10081 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
    10141 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
    10201 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
    10261 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    10321 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    10381 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    10441 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    10501 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
    10561 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    10621 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    10681 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    10741 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    10801 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    10861 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    10921 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10981 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    11041 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    11101 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    11161 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    11221 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    11281 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    11341 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    11401 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    11461 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    11521 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    11581 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    11641 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    11701 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    11761 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    11821 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    11881 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    11941 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    12001 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    12061 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    12121 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    12181 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    12241 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    12301 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    12361 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    12421 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    12481 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    12541 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    12601 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    12661 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    12721 c
//
