LOCUS       sequence-129461-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_763_4xUAS_UPS_hkCP
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    244..268
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(373..397)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    411..502
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(443..462)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    516..564
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(832..853)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc attgttctgc ggttaaggat
       61 gcactgcact ggaaaagata gttaattgtt catatttatt acattttcga taagaaaacc
      121 tcttcatttc ggagaaataa aatccaagca tctgtacctt ttgacaaccg gaaagaaaga
      181 gaacaaaatc ggcatggcgc tggagatgtg agtttttagc tattggtaca agattgcaga
      241 tctaccactt tgtacaagaa agctgggtcc tcggaggaca gtactccgct cggaggacag
      301 tactccgacc tgcaggcatg caagcttccc gctcggagga cagtactccg ctcggaggac
      361 agtactcccg gaagcctgct tttttgtaca aacttgtggt acctatcgat agagaaatgt
      421 tctggcacct gcacttgcac tggggacagc ctattttgct agtttgtttt gtttcgtttt
      481 gttttgatgg agagcgtatg ttagtactat cgattcacac aaaaaaccaa cacacagatg
      541 taatgaaaat aaagatattt tattgcggcc gctccaagta cctcccgtac cttaatatta
      601 cttacttatc atggtagctt gggctggcgt aatagcgaag aggcccgcac cgatcgccct
      661 tcccaacagt tgcgcagcct gaatggcgaa tggcaaattg taagcgttaa tattttgtta
      721 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
      781 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
      841 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
      901 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
      961 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     1021 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag ga
//
