LOCUS       sequence-129463-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd gen lentiviral vector. tet/dox controllable expression of 
            FLAG-HA-tagged murine Dnd1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pCS-TRE-FHDnd1-Ubc-rtTA-I2G
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(124..741,742..747,748..783,784..786,787..822,823..825,
                     826..861,862..870)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag
       61 actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga caagcttgtc
      121 accatgtcta gactggacaa gagcaaagtc ataaacggcg ctctggaatt actcaatgga
      181 gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct
      241 accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg
      301 ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg
      361 cggaacaacg ccaagtcatt ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg
      421 catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc
      481 ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac
      541 tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag
      601 acacctacca ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg
      661 cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa
      721 cagctaaagt gcgaaagcgg cgggccggcc gacgcccttg acgattttga cttagacatg
      781 ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac
      841 gattttgacc ttgacatgct ccccgggtaa ctcgagatcc gcccctctcc ctc
//
