LOCUS       sequence-129474-        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_711_OSC-fkh_dCP
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    236..260
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(765..789)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    803..894
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(835..854)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    908..956
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 tggcgccggg cctttcttta tgtttttggc gtcttccatg gtggctttac caacagtacc
       61 ggaatgccgt ttggtatgcg tcttgtgatt caaagttggc ttattcaaag gatattaacg
      121 aaggcagcgg cacgtctgct cggctcgaac attcattcac aactgatgct ctcagccacc
      181 ccgcgccctt ttataccgct gcgctcgatc cccgggcgag ctcgatcgca gatctaccac
      241 tttgtacaag aaagctgggt cgaattcgcc cttagaatgc tcagtttttc atagtattca
      301 aattgtattc tattttgtcc ccatgccaca ttcatattca tatctgggtt ttagaaacca
      361 ccgagtgggt ctcagcttgc atcatagatt ttgggtttca tctcgccgtt ttccgagaag
      421 tggcatcttc tgtgtggtct gtcggcatgg ataaatgggc ttccagcgac gacgaccccc
      481 gcgggcaaca gtttaagaat caaacggagt actgtcctcc gagcttcgat cccgatccct
      541 ctggacttcg ataagttatg tcaacaaact tcggtcgcgt aacggcgcat gcaggagctc
      601 tggagtagta ggagctccag ctgaagtgca gcattgggtt acaatcgtcc acatggagga
      661 gatcagcatc agcccctagg agggggaggg ggcggtgttg gaactttgga gcagtccagc
      721 attcagttgg ggcggcgtaa ttcggcaata aagggcgaat tcggagcctg cttttttgta
      781 caaacttgtg gtacctatcg atagagaaat gttctggcac ctgcacttgc actggggaca
      841 gcctattttg ctagtttgtt ttgtttcgtt ttgttttgat ggagagcgta tgttagtact
      901 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
      961 ccgctccaag tacctcccgt accttaatat tacttactta tcatggtagc ttgggctggc
     1021 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     1081 aatg
//
