LOCUS       sequence-129477-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_770_sn-EcR_dCP
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..50
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(569..593)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      784..793
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(856..874)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gaacatttct ctatcgatag gtaccacaag tttgtacaaa aaagcaggct tctggcatta
       61 tcaatgcaaa tgggcatggt attttaaaat cttggaccgg cgggtgtgtt caaggcgccc
      121 gcctgtcgtc gtttctgctg cacttggtcc tacccacttc cagttcccat tcccattcca
      181 atttccattc ccattccctt gcagcgattg cagtacaaat aattgatatg ctctctcggt
      241 cctttgccgt cagacttttc agatttttct gccgacgata ttttgtgcgg agtactgtcc
      301 tccgagtcgg catcagcagg cccaaacata tcagcatttc cgttcgataa agtgaaatgc
      361 atatttataa aaccgaatta tcaacagaac taactcccat cagccaaaga gaagcacttg
      421 gcctagccag ataattgaca atcgaaagga acaatgaatg tggtcggagt actgtcctcc
      481 gagtatacga acaaaaacat ttacaacaat agcttgtggc acattcattt tgaaaagaat
      541 attacttact gaaattatct ccaatgggac ccagctttct tgtacaaagt ggtagatctg
      601 cgatcgagct cgcccgggga tcgagcgcag cggtataaaa gggcgcgggg tggctgagag
      661 catcagttgt gaatgaatgt tcgagccgag cagacgtgcc gctgccttcg ttaatatcct
      721 ttgaataagc caactttgaa tcacaagacg cataccaaac ggcattccgg tactgttggt
      781 aaagccacca tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg
      841 gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct
      901 ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc
      961 gaaatgtccg ttcggttggc agaagctatg aaa
//
