LOCUS       sequence-129478-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_770_sn-EcR_dCP
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    237..261
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(780..804)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    818..909
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(850..869)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggcttta ccaacagtac
       61 cggaatgccg tttggtatgc gtcttgtgat tcaaagttgg cttattcaaa ggatattaac
      121 gaaggcagcg gcacgtctgc tcggctcgaa cattcattca caactgatgc tctcagccac
      181 cccgcgccct tttataccgc tgcgctcgat ccccgggcga gctcgatcgc agatctacca
      241 ctttgtacaa gaaagctggg tcccattgga gataatttca gtaagtaata ttcttttcaa
      301 aatgaatgtg ccacaagcta ttgttgtaaa tgtttttgtt cgtatactcg gaggacagta
      361 ctccgaccac attcattgtt cctttcgatt gtcaattatc tggctaggcc aagtgcttct
      421 ctttggctga tgggagttag ttctgttgat aattcggttt tataaatatg catttcactt
      481 tatcgaacgg aaatgctgat atgtttgggc ctgctgatgc cgactcggag gacagtactc
      541 cgcacaaaat atcgtcggca gaaaaatctg aaaagtctga cggcaaagga ccgagagagc
      601 atatcaatta tttgtactgc aatcgctgca agggaatggg aatggaaatt ggaatgggaa
      661 tgggaactgg aagtgggtag gaccaagtgc agcagaaacg acgacaggcg ggcgccttga
      721 acacacccgc cggtccaaga ttttaaaata ccatgcccat ttgcattgat aatgccagaa
      781 gcctgctttt ttgtacaaac ttgtggtacc tatcgataga gaaatgttct ggcacctgca
      841 cttgcactgg ggacagccta ttttgctagt ttgttttgtt tcgttttgtt ttgatggaga
      901 gcgtatgtta gtactatcga ttcacacaaa aaaccaacac acagatgtaa tgaaaat
//
