LOCUS       sequence-129479-        1119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_769_DipB-EcR_dCP
FEATURES             Location/Qualifiers
     source          1..1119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..50
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(350..374)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      565..574
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(637..655)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gaacatttct ctatcgatag gtaccacaag tttgtacaaa aaagcaggct tctcgttagc
       61 tccatctccg gagtactgtc ctccgagata atcattatct gctgcctgca gctcaatcag
      121 ctcagttcag tcctcttcag atctgctcag ttgttgccgc tccattataa tggagtaatt
      181 atggcgtgct cgagagccaa gaattttctg cactggacct caatgccaaa caattccctt
      241 ttgtcatgat atggccttat ctgttactct gttactccaa ccatagttca gctttcctga
      301 taacggagta ctgtcctccg agcattggcg ctcgactttg tttaccacga cccagctttc
      361 ttgtacaaag tggtagatct gcgatcgagc tcgcccgggg atcgagcgca gcggtataaa
      421 agggcgcggg gtggctgaga gcatcagttg tgaatgaatg ttcgagccga gcagacgtgc
      481 cgctgccttc gttaatatcc tttgaataag ccaactttga atcacaagac gcataccaaa
      541 cggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat aaagaaaggc
      601 ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg
      661 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac
      721 atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat
      781 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg
      841 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat
      901 gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa
      961 aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt
     1021 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct
     1081 catctacctc ccggttttaa tgaatacgat tttgtgcca
//
