LOCUS       sequence-129480-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_769_DipB-EcR_dCP
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    239..263
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(563..587)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    601..692
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(633..652)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    706..754
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc atggtggctt taccaacagt
       61 accggaatgc cgtttggtat gcgtcttgtg attcaaagtt ggcttattca aaggatatta
      121 acgaaggcag cggcacgtct gctcggctcg aacattcatt cacaactgat gctctcagcc
      181 accccgcgcc cttttatacc gctgcgctcg atccccgggc gagctcgatc gcagatctac
      241 cactttgtac aagaaagctg ggtcgtggta aacaaagtcg agcgccaatg ctcggaggac
      301 agtactccgt tatcaggaaa gctgaactat ggttggagta acagagtaac agataaggcc
      361 atatcatgac aaaagggaat tgtttggcat tgaggtccag tgcagaaaat tcttggctct
      421 cgagcacgcc ataattactc cattataatg gagcggcaac aactgagcag atctgaagag
      481 gactgaactg agctgattga gctgcaggca gcagataatg attatctcgg aggacagtac
      541 tccggagatg gagctaacga gaagcctgct tttttgtaca aacttgtggt acctatcgat
      601 agagaaatgt tctggcacct gcacttgcac tggggacagc ctattttgct agtttgtttt
      661 gtttcgtttt gttttgatgg agagcgtatg ttagtactat cgattcacac aaaaaaccaa
      721 cacacagatg taatgaaaat aaagatattt tattgcggcc gctccaagta cctcccgtac
      781 cttaatatta cttacttatc atggtagctt gggctggcgt aatagcgaag aggcccgcac
      841 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcaaattg taagcgttaa
      901 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
      961 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     1021 tccagtttgg
//
