LOCUS       sequence-129483-        1131 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_768_Nhe2-EcR_dCP
FEATURES             Location/Qualifiers
     source          1..1131
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..50
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(493..517)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      708..717
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(780..798)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gaacatttct ctatcgatag gtaccacaag tttgtacaaa aaagcaggct tcgttttcac
       61 ggagtactgt cctccgagtg tcaacactct gtaatccgga gtactgtcct ccgagaaatt
      121 aaaacaccaa attgaatttg tggaaggcct aggccactgg ttagaacaat tgaatatgga
      181 gacggaaaaa tgcatgaggc atcatcctaa attgactacc aattttatat aatgagatgg
      241 gcgacactca ttccagcaga tagcgaatac caacggatgc aattgctcaa gtcgggacaa
      301 ttactgaatc cgaccggagt actgtcctcc gagagagatt tatcagcatc tcgaaaatta
      361 cactcgatgt acacaccaat tgctcaattg ggagtcttta gtcattccgc actcgacaga
      421 taagctttcg ctactgctgc taataaaatt tcgccaataa tgcaaatcag atcttatcgc
      481 aggccaactg ggacccagct ttcttgtaca aagtggtaga tctgcgatcg agctcgcccg
      541 gggatcgagc gcagcggtat aaaagggcgc ggggtggctg agagcatcag ttgtgaatga
      601 atgttcgagc cgagcagacg tgccgctgcc ttcgttaata tcctttgaat aagccaactt
      661 tgaatcacaa gacgcatacc aaacggcatt ccggtactgt tggtaaagcc accatggaag
      721 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg
      781 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta
      841 cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt
      901 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg
      961 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg
     1021 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc
     1081 ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg c
//
