LOCUS       sequence-129484-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_768_Nhe2-EcR_dCP
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    239..263
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(706..730)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    744..835
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(776..795)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    849..897
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc atggtggctt taccaacagt
       61 accggaatgc cgtttggtat gcgtcttgtg attcaaagtt ggcttattca aaggatatta
      121 acgaaggcag cggcacgtct gctcggctcg aacattcatt cacaactgat gctctcagcc
      181 accccgcgcc cttttatacc gctgcgctcg atccccgggc gagctcgatc gcagatctac
      241 cactttgtac aagaaagctg ggtcccagtt ggcctgcgat aagatctgat ttgcattatt
      301 ggcgaaattt tattagcagc agtagcgaaa gcttatctgt cgagtgcgga atgactaaag
      361 actcccaatt gagcaattgg tgtgtacatc gagtgtaatt ttcgagatgc tgataaatct
      421 ctctcggagg acagtactcc ggtcggattc agtaattgtc ccgacttgag caattgcatc
      481 cgttggtatt cgctatctgc tggaatgagt gtcgcccatc tcattatata aaattggtag
      541 tcaatttagg atgatgcctc atgcattttt ccgtctccat attcaattgt tctaaccagt
      601 ggcctaggcc ttccacaaat tcaatttggt gttttaattt ctcggaggac agtactccgg
      661 attacagagt gttgacactc ggaggacagt actccgtgaa aacgaagcct gcttttttgt
      721 acaaacttgt ggtacctatc gatagagaaa tgttctggca cctgcacttg cactggggac
      781 agcctatttt gctagtttgt tttgtttcgt tttgttttga tggagagcgt atgttagtac
      841 tatcgattca cacaaaaaac caacacacag atgtaatgaa aataaagata ttttattgcg
      901 gccgctccaa gtacctcccg taccttaata ttacttactt atcatggtag cttgggctgg
      961 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     1021 gaatggcaaa ttgtaagcgt taatattt
//
