LOCUS       sequence-129495-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses K227M nickase variant of I-Ani I nuclease in mammalian 
            cells with BFP tracker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             65..91
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             95..115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cccctcactc ggcgcgccag tcctccgaca gactgagtcg cccgctcgag ccgccaccat
       61 gggatatcca tacgatgtcc cagattatgc gccacctaag aagaaacgca aagtcgaatt
      121 cggcagcgat ctgacctacg cctatctggt gggcctgtac gagggcgacg gatatttttc
      181 catcactaaa aagggcaagt acctgaccta tgagctggga attgaactgt ctatcaagga
      241 tgtgcagctg atctacaaga tcaagaagat cctggggatc ggcattgtga gcttcaggaa
      301 gagaaacgag attgaaatgg tggccctgag gatcagggat aagaatcacc tgaaatctaa
      361 gattctgccc atcttcgaga agtatcctat gtttagtaac aaacagtacg actatctgag
      421 gtttagaaat gctctgctga gcggcatcat ctacctggag gatctgccag actatacccg
      481 gtccgacgag cccctgaaca gcatcgaatc catcattaat acatcttact tcagtgcctg
      541 gctggtgggc ttcatcgagg ctgaagggtg cttctctgtg tacaaactga acaaggacga
      601 tgactatctg attgccagtt ttgatatcgc tcagagggat ggagacatcc tgattagcgc
      661 catcagaaag tacctgtcct tcaccacaaa ggtgtatctg gacaaaacaa attgtagcaa
      721 actgaaggtc actagcgtgc gctccgtgga gaacatcatt aagttcctgc agaatgctcc
      781 tgtgaaactg ctgggcaaca tgaagctgca gtacaaactg tggctgaagc agctgcggaa
      841 aatctctcgc tacagtgaaa aaatcaagat tccatccaat tattaacctg caggtcgagc
      901 atgcatctag ggcggccaat tccgcccct
//
