LOCUS       sequence-129505-        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codes for the Active/Repressed TLR with the target site for I-Sce I 
            nuclease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pCVL.Active/Repressed TLR (Sce)
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     233..254
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(297..350)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
       61 tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggttcacac tagttaggga
      121 taacagggta atacctgcag gttgccggtg gtgcagatga acttcagggt cagcttgccg
      181 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
      241 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcacgggg
      301 cccggattct cctccacgtc accgcatgtt agaagacttc ctctgccctc tctagactct
      361 tccatcacgc cgatctgcca ggccagaacc tgggcgacta gctcgcaggc ttggcggcca
      421 tcgagatcga cgtagtacgg cgttcggtga tggcaaacga tcaatcccca cagtcgctcg
      481 ccgcgcaaaa tcgagatcga catcgtgccg tgcatgccta tgttgcgcat gaattccaga
      541 tggacgggcg agacgctgcg caggatggcg aagctaagat caatcggccg cccggtgacc
      601 ggattgaggt ctggggtgac cggcaccggc cgataattga tatcgggaat gatccgtacc
      661 gggttgatgg tatagagccg acgggcctgc gccggcacgg ttgaggcagg atagtgcagg
      721 cctagttttg actcgacctc ggcgcaccga tcctctgcga tcacttcgcc gctgaagtcg
      781 gaggcgaagc gatagatcat cacccgatcg aagccggtaa tcttccgcac ctcttgcgcc
      841 gcggcggcgc aggcgctttc caaggtttcg gcggcctgca ggcggcggat ggcgctgttg
      901 gtgcggcgga agaacgcctg cggctcggcg acgtcccgct ggggaggctc gagctcgagg
      961 aagatgagct gatcatggcg atgccaggag ccgatgaagc ctgcgtcctt tcgcatcgtg
     1021 aagccgacag tgatcggtgc tccgacggcc gccccgggct cggccaaggc ga
//
