LOCUS       sequence-129509-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tetracycline-inducible expression vector for Staphylococcus aureus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Corrigan RM, Foster TJ
  TITLE     An improved tetracycline-inducible expression vector for 
            Staphylococcus aureus.
  JOURNAL   Plasmid. 2009 Mar;61(2):126-9. doi: 10.1016/j.plasmid.2008.10.001. 
            Epub 2008 Nov 25.
  PUBMED    18996145
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2008.10.001"; journalName: "Plasmid"; date: 
            "2009-03"; volume: "61"; issue: "2"; pages: "126-9"
COMMENT     Sequence Label: pRMC2
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..78
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     189..205
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    256..274
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     primer_bind     complement(457..476)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
       61 tgtaaaacga cggccagtga attcgagctc agatctgtta acggtaccat caagcttatt
      121 ttaattatac tcatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
      181 acgacgttgt aaaacgacgg ccagtgaatt cgagctcaga tctgttaacg gtaccatcaa
      241 gcttatttta attatactct atcaatgata gagtgtcaat atttttttta gtttttcatg
      301 aactcgaggg gatccaaata aaaaactagt ttgacaaata actctatcaa tgatataatg
      361 tcaacaaaaa ggaggaatta atgatgtcta gattagataa aagtaaagtg attaacagcg
      421 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
      481 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg
      541 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg
      601 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc
      661 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc
      721 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg
      781 cactcagcgc tgt
//
