LOCUS       sequence-129512-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tetracycline-inducible expression vector for Staphylococcus aureus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Corrigan RM, Foster TJ
  TITLE     An improved tetracycline-inducible expression vector for 
            Staphylococcus aureus.
  JOURNAL   Plasmid. 2009 Mar;61(2):126-9. doi: 10.1016/j.plasmid.2008.10.001. 
            Epub 2008 Nov 25.
  PUBMED    18996145
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2008.10.001"; journalName: "Plasmid"; date: 
            "2009-03"; volume: "61"; issue: "2"; pages: "126-9"
COMMENT     Sequence Label: pRMC2
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    34..52
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     primer_bind     complement(103..119)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(230..246)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(455..474)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     574..596
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(634..652)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgagttcat gaaaaactaa aaaaaatatt gacactctat cattgataga gtataattaa
       61 aataagcttg atggtaccgt taacagatct gagctcgaat tcactggccg tcgttttaca
      121 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatgagta
      181 taattaaaat aagcttgatg gtaccgttaa cagatctgag ctcgaattca ctggccgtcg
      241 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      301 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      361 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
      421 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
      481 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
      541 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
      601 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
      661 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      721 gcggaacccc tatttgttta ttttt
//
